TY - JOUR
T1 - Plasma membrane calcium ATPase expression in human colon multistep carcinogenesis
AU - Rüschoff, Jan H.
AU - Brandenburger, Timo
AU - Strehler, Emanuel E.
AU - Filoteo, Adelaida G.
AU - Heinmöller, Ernst
AU - Aumüller, Gerhard
AU - Wilhelm, Beate
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/5
Y1 - 2012/5
N2 - The expression of the plasma membrane Ca 2+ ATPase (PMCA) was analyzed in a series of 84 formalin-fixed and paraffin embedded colon samples including normal mucosa (n = 32), adenoma (n = 19), adenocarcinoma (n = 27), and lymph node metastasis (n = 6) using (i) immunohistochemistry, (ii) mRNA in situ hybridization, and (iii) quantitative reverse-transcriptase PCR. A marked reduction of PMCA4 protein was observed in high-grade adenoma, colon cancer as well as lymph node metastasis, pointing to its potential role in the progression of cancer. However, PMCA4 RNA transcripts were unchanged or even increased in colon carcinomas, suggesting posttranscriptional regulation of PMCA4 during carcinogenesis.
AB - The expression of the plasma membrane Ca 2+ ATPase (PMCA) was analyzed in a series of 84 formalin-fixed and paraffin embedded colon samples including normal mucosa (n = 32), adenoma (n = 19), adenocarcinoma (n = 27), and lymph node metastasis (n = 6) using (i) immunohistochemistry, (ii) mRNA in situ hybridization, and (iii) quantitative reverse-transcriptase PCR. A marked reduction of PMCA4 protein was observed in high-grade adenoma, colon cancer as well as lymph node metastasis, pointing to its potential role in the progression of cancer. However, PMCA4 RNA transcripts were unchanged or even increased in colon carcinomas, suggesting posttranscriptional regulation of PMCA4 during carcinogenesis.
KW - Colorectal cancer
KW - PMCA
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=84860167304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860167304&partnerID=8YFLogxK
U2 - 10.3109/07357907.2012.657817
DO - 10.3109/07357907.2012.657817
M3 - Article
C2 - 22480210
AN - SCOPUS:84860167304
SN - 0735-7907
VL - 30
SP - 251
EP - 257
JO - Cancer Investigation
JF - Cancer Investigation
IS - 4
ER -