Plasma membrane calcium ATPase expression in human colon multistep carcinogenesis

Jan H. Rüschoff, Timo Brandenburger, Emanuel E. Strehler, Adelaida G. Filoteo, Ernst Heinmöller, Gerhard Aumüller, Beate Wilhelm

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The expression of the plasma membrane Ca 2+ ATPase (PMCA) was analyzed in a series of 84 formalin-fixed and paraffin embedded colon samples including normal mucosa (n = 32), adenoma (n = 19), adenocarcinoma (n = 27), and lymph node metastasis (n = 6) using (i) immunohistochemistry, (ii) mRNA in situ hybridization, and (iii) quantitative reverse-transcriptase PCR. A marked reduction of PMCA4 protein was observed in high-grade adenoma, colon cancer as well as lymph node metastasis, pointing to its potential role in the progression of cancer. However, PMCA4 RNA transcripts were unchanged or even increased in colon carcinomas, suggesting posttranscriptional regulation of PMCA4 during carcinogenesis.

Original languageEnglish (US)
Pages (from-to)251-257
Number of pages7
JournalCancer Investigation
Volume30
Issue number4
DOIs
StatePublished - May 2012

Keywords

  • Colorectal cancer
  • PMCA
  • Signal transduction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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