Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction

Marianela G. Dalghi, Marisa M. Fernández, Mariela Ferreira-Gomes, Irene C. Mangialavori, Emilio L. Malchiodi, Emanuel E. Strehler, Juan Pablo F C Rossi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Plasma membrane calcium ATPases interact dynamically with the submembrane actin cytoskeleton. Results: Biophysical and functional assays show that purified plasma membrane calcium ATPase binds to G-actin and is activated by short actin oligomers. Conclusion: Plasma membrane calcium ATPases are regulated by polymerizing actin independently of regulation by calmodulin. Significance: Dynamic actin participates in cytosolic Ca2+ homeostasis by regulating plasma membrane calcium ATPase activity.

Original languageEnglish (US)
Pages (from-to)23380-23393
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number32
DOIs
StatePublished - Aug 9 2013

Fingerprint

Plasma Membrane Calcium-Transporting ATPases
Oligomers
Actins
Calmodulin
Actin Cytoskeleton
Homeostasis
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Dalghi, M. G., Fernández, M. M., Ferreira-Gomes, M., Mangialavori, I. C., Malchiodi, E. L., Strehler, E. E., & Rossi, J. P. F. C. (2013). Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction. Journal of Biological Chemistry, 288(32), 23380-23393. https://doi.org/10.1074/jbc.M113.470542

Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction. / Dalghi, Marianela G.; Fernández, Marisa M.; Ferreira-Gomes, Mariela; Mangialavori, Irene C.; Malchiodi, Emilio L.; Strehler, Emanuel E.; Rossi, Juan Pablo F C.

In: Journal of Biological Chemistry, Vol. 288, No. 32, 09.08.2013, p. 23380-23393.

Research output: Contribution to journalArticle

Dalghi, MG, Fernández, MM, Ferreira-Gomes, M, Mangialavori, IC, Malchiodi, EL, Strehler, EE & Rossi, JPFC 2013, 'Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction', Journal of Biological Chemistry, vol. 288, no. 32, pp. 23380-23393. https://doi.org/10.1074/jbc.M113.470542
Dalghi MG, Fernández MM, Ferreira-Gomes M, Mangialavori IC, Malchiodi EL, Strehler EE et al. Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction. Journal of Biological Chemistry. 2013 Aug 9;288(32):23380-23393. https://doi.org/10.1074/jbc.M113.470542
Dalghi, Marianela G. ; Fernández, Marisa M. ; Ferreira-Gomes, Mariela ; Mangialavori, Irene C. ; Malchiodi, Emilio L. ; Strehler, Emanuel E. ; Rossi, Juan Pablo F C. / Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 32. pp. 23380-23393.
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