Plasma immune analytes in patients with epithelial ovarian cancer

Matthew S Block, Matthew J. Maurer, Krista Goergen, Kimberly R. Kalli, Courtney L. Erskine, Marshall D. Behrens, Ann L Oberg, Keith L Knutson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives: Inflammation is a common feature of epithelial ovarian cancer (EOC), and measurement of plasma markers of inflammation might identify candidate markers for use in screening or presurgical evaluation of patients with adnexal masses. Methods: Plasma specimens from cohorts of 100 patients with advanced EOC (AJCC Stage III and IV), 50 patients with early stage EOC (Stage I and II), and 50 patients with benign surgical conditions were assayed for concentrations of multiple cytokines, toll-like receptor agonists, and vascular growth factors via ELISA and electrochemiluminescence. Immune proteins were then analyzed for association with EOC. Differences in plasma protein levels between benign, early, and advanced EOC patient groups were assessed with and without adjustment for plasma cancer antigen 125 (CA-125) levels. Results: Out of 23 proteins tested, six-including interferon gamma (IFNγ), interleukin 6 (IL-6), IL-8, IL-10, tumor necrosis factor alpha (TNFα), and placental growth factor (PlGF)-were univariately associated with EOC (all p<. 0.005), and one-IL-6-was associated with early stage EOC (. p<. 0.0001). Heat shock protein 90. kDa beta member 1 (HSP90B1, gp96) was associated with EOC and early stage EOC with borderline statistical significance (. p=. 0.039 and p=. 0.026, respectively). However, when adjusted for (CA-125), only HSP90B1 independently predicted EOC (. p=. 0.008), as well as early stage EOC (. p=. 0.014). Conclusions: Multiple plasma cytokines, including IFNγ, IL-6, IL-8, IL-10, TNFα, PlGF, and HSP90B1 are associated with EOC. Of these, HSP90B1 is associated with EOC independent from the biomarker CA-125.

Original languageEnglish (US)
Pages (from-to)108-113
Number of pages6
JournalCytokine
Volume73
Issue number1
DOIs
StatePublished - May 1 2015

Fingerprint

Plasmas
Interleukin-6
Intercellular Signaling Peptides and Proteins
Interleukin-8
Antigens
Interleukin-10
Interferon-gamma
Tumor Necrosis Factor-alpha
Cytokines
HSP90 Heat-Shock Proteins
Toll-Like Receptors
Tumor Biomarkers
Blood Proteins
Screening
Proteins
Ovarian epithelial cancer
Inflammation
Neoplasms
Enzyme-Linked Immunosorbent Assay

Keywords

  • HSP90B1
  • Interleukin 6 (IL-6)
  • Ovarian cancer

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Block, M. S., Maurer, M. J., Goergen, K., Kalli, K. R., Erskine, C. L., Behrens, M. D., ... Knutson, K. L. (2015). Plasma immune analytes in patients with epithelial ovarian cancer. Cytokine, 73(1), 108-113. https://doi.org/10.1016/j.cyto.2015.01.035

Plasma immune analytes in patients with epithelial ovarian cancer. / Block, Matthew S; Maurer, Matthew J.; Goergen, Krista; Kalli, Kimberly R.; Erskine, Courtney L.; Behrens, Marshall D.; Oberg, Ann L; Knutson, Keith L.

In: Cytokine, Vol. 73, No. 1, 01.05.2015, p. 108-113.

Research output: Contribution to journalArticle

Block, MS, Maurer, MJ, Goergen, K, Kalli, KR, Erskine, CL, Behrens, MD, Oberg, AL & Knutson, KL 2015, 'Plasma immune analytes in patients with epithelial ovarian cancer', Cytokine, vol. 73, no. 1, pp. 108-113. https://doi.org/10.1016/j.cyto.2015.01.035
Block MS, Maurer MJ, Goergen K, Kalli KR, Erskine CL, Behrens MD et al. Plasma immune analytes in patients with epithelial ovarian cancer. Cytokine. 2015 May 1;73(1):108-113. https://doi.org/10.1016/j.cyto.2015.01.035
Block, Matthew S ; Maurer, Matthew J. ; Goergen, Krista ; Kalli, Kimberly R. ; Erskine, Courtney L. ; Behrens, Marshall D. ; Oberg, Ann L ; Knutson, Keith L. / Plasma immune analytes in patients with epithelial ovarian cancer. In: Cytokine. 2015 ; Vol. 73, No. 1. pp. 108-113.
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abstract = "Objectives: Inflammation is a common feature of epithelial ovarian cancer (EOC), and measurement of plasma markers of inflammation might identify candidate markers for use in screening or presurgical evaluation of patients with adnexal masses. Methods: Plasma specimens from cohorts of 100 patients with advanced EOC (AJCC Stage III and IV), 50 patients with early stage EOC (Stage I and II), and 50 patients with benign surgical conditions were assayed for concentrations of multiple cytokines, toll-like receptor agonists, and vascular growth factors via ELISA and electrochemiluminescence. Immune proteins were then analyzed for association with EOC. Differences in plasma protein levels between benign, early, and advanced EOC patient groups were assessed with and without adjustment for plasma cancer antigen 125 (CA-125) levels. Results: Out of 23 proteins tested, six-including interferon gamma (IFNγ), interleukin 6 (IL-6), IL-8, IL-10, tumor necrosis factor alpha (TNFα), and placental growth factor (PlGF)-were univariately associated with EOC (all p<. 0.005), and one-IL-6-was associated with early stage EOC (. p<. 0.0001). Heat shock protein 90. kDa beta member 1 (HSP90B1, gp96) was associated with EOC and early stage EOC with borderline statistical significance (. p=. 0.039 and p=. 0.026, respectively). However, when adjusted for (CA-125), only HSP90B1 independently predicted EOC (. p=. 0.008), as well as early stage EOC (. p=. 0.014). Conclusions: Multiple plasma cytokines, including IFNγ, IL-6, IL-8, IL-10, TNFα, PlGF, and HSP90B1 are associated with EOC. Of these, HSP90B1 is associated with EOC independent from the biomarker CA-125.",
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AB - Objectives: Inflammation is a common feature of epithelial ovarian cancer (EOC), and measurement of plasma markers of inflammation might identify candidate markers for use in screening or presurgical evaluation of patients with adnexal masses. Methods: Plasma specimens from cohorts of 100 patients with advanced EOC (AJCC Stage III and IV), 50 patients with early stage EOC (Stage I and II), and 50 patients with benign surgical conditions were assayed for concentrations of multiple cytokines, toll-like receptor agonists, and vascular growth factors via ELISA and electrochemiluminescence. Immune proteins were then analyzed for association with EOC. Differences in plasma protein levels between benign, early, and advanced EOC patient groups were assessed with and without adjustment for plasma cancer antigen 125 (CA-125) levels. Results: Out of 23 proteins tested, six-including interferon gamma (IFNγ), interleukin 6 (IL-6), IL-8, IL-10, tumor necrosis factor alpha (TNFα), and placental growth factor (PlGF)-were univariately associated with EOC (all p<. 0.005), and one-IL-6-was associated with early stage EOC (. p<. 0.0001). Heat shock protein 90. kDa beta member 1 (HSP90B1, gp96) was associated with EOC and early stage EOC with borderline statistical significance (. p=. 0.039 and p=. 0.026, respectively). However, when adjusted for (CA-125), only HSP90B1 independently predicted EOC (. p=. 0.008), as well as early stage EOC (. p=. 0.014). Conclusions: Multiple plasma cytokines, including IFNγ, IL-6, IL-8, IL-10, TNFα, PlGF, and HSP90B1 are associated with EOC. Of these, HSP90B1 is associated with EOC independent from the biomarker CA-125.

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