Plasma extracellular RNA profiles in healthy and cancer patients

Tiezheng Yuan, Xiaoyi Huang, Mark Woodcock, Meijun Du, Rachel Dittmar, Yuan Wang, Susan Tsai, Manish Kohli, Lisa Allyn Boardman, Tushar C Patel, Liang Wang

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

Extracellular vesicles are selectively enriched in RNA that has potential as disease biomarkers. To systemically characterize circulating extracellular RNA (exRNA) profiles, we performed RNA sequencing analysis on plasma extracellular vesicles derived from 50 healthy individuals and 142 cancer patients. Of ∼12.6 million raw reads for each individual, the number of mappable reads aligned to RNA references was ∼5.4 million including miRNAs (∼40.4%), piwiRNAs (∼40.0%), pseudo-genes (∼3.7%), lncRNAs (∼2.4%), tRNAs (∼2.1%), and mRNAs (∼2.1%). By expression stability testing, we identified a set of miRNAs showing relatively consistent expression, which may serve as reference control for exRNA quantification. By performing multivariate analysis of covariance, we identified significant associations of these exRNAs with age, sex and different types of cancers. In particular, down-regulation of miR-125a-5p and miR-1343-3p showed an association with all cancer types tested (false discovery rate

Original languageEnglish (US)
Article number19413
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 20 2016

ASJC Scopus subject areas

  • General

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    Yuan, T., Huang, X., Woodcock, M., Du, M., Dittmar, R., Wang, Y., Tsai, S., Kohli, M., Boardman, L. A., Patel, T. C., & Wang, L. (2016). Plasma extracellular RNA profiles in healthy and cancer patients. Scientific Reports, 6, [19413]. https://doi.org/10.1038/srep19413