PKCδ null mutations in a mouse model of osteoarthritis alter osteoarthritic pain independently of joint pathology by augmenting NGF/TrkA-induced axonal outgrowth

Ranjan Kc, Xin Li, Jeffrey S. Kroin, Zhiqiang Liu, Di Chen, Guozhi Xiao, Brett Levine, Jinyuan Li, John L. Hamilton, Andre J. Van Wijnen, Margaret Piel, Daniel A. Shelly, Dovrat Brass, Ela Kolb, Hee Jeong Im

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Objectives: A key clinical paradox in osteoarthritis (OA), a prevalent age-related joint disorder characterised by cartilage degeneration and debilitating pain, is that the severity of joint pain does not strictly correlate with radiographic and histological defects in joint tissues. Here, we determined whether protein kinase Cδ (PKCδ), a key mediator of cartilage degeneration, is critical to the mechanism by which OA develops from an asymptomatic joint-degenerative condition to a painful disease. Methods: OA was induced in 10-week-old PKCδ null (PKCδ-/-) and wild-type mice by destabilisation of the medial meniscus (DMM) followed by comprehensive examination of the histology, molecular pathways and knee-pain-related-behaviours in mice, and comparisons with human biopsies. Results: In the DMM model, the loss of PKCδ expression prevented cartilage degeneration but exacerbated OA-associated hyperalgesia. Cartilage preservation corresponded with reduced levels of inflammatory cytokines and of cartilage-degrading enzymes in the joints of PKCδ-deficient DMM mice. Hyperalgesia was associated with stimulation of nerve growth factor (NGF) by fibroblast-like synovial cells and with increased synovial angiogenesis. Results: from tissue specimens of patients with symptomatic OA strikingly resembled our findings from the OA animal model. In PKCδ null mice, increases in sensory neuron distribution in knee OA synovium and activation of the NGF-tropomyosin receptor kinase (TrkA) axis in innervating dorsal root ganglia were highly correlated with knee OA hyperalgesia. Conclusions: Increased distribution of synovial sensory neurons in the joints, and augmentation of NGF/TrkA signalling, causes OA hyperalgesia independently of cartilage preservation.

Original languageEnglish (US)
Pages (from-to)2133-2141
Number of pages9
JournalAnnals of the rheumatic diseases
Volume75
Issue number12
DOIs
StatePublished - Dec 1 2016

Keywords

  • Chondrocytes
  • Fibroblasts
  • Knee Osteoarthritis
  • Synovitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

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    Kc, R., Li, X., Kroin, J. S., Liu, Z., Chen, D., Xiao, G., Levine, B., Li, J., Hamilton, J. L., Van Wijnen, A. J., Piel, M., Shelly, D. A., Brass, D., Kolb, E., & Im, H. J. (2016). PKCδ null mutations in a mouse model of osteoarthritis alter osteoarthritic pain independently of joint pathology by augmenting NGF/TrkA-induced axonal outgrowth. Annals of the rheumatic diseases, 75(12), 2133-2141. https://doi.org/10.1136/annrheumdis-2015-208444