Abstract
Platelets have been regarded as static cells that do not move once they adhere to a matrix. The present study explored, whether platelets are able to migrate. In contrast to the current opinion, we found that platelets were mobile, able to migrate over a surface, and transmigrate through a transwell membrane and endothelium toward a source of stromal cellderived factor 1 (SDF-1). Platelet migration was stimulated by SDF-1, which led to the downstream activation and phosphorylation of Wiskott-Aldrich syndrome protein. SDF-1 signaling and subsequent platelet migration could be inhibited by CXCR4-receptor blocker AMD3100, pertussis toxin, inhibition of phosphoinositol 3-kinase (PI3 kinase) with LY294002 or wortmannin, and disruption of actin polymerization with cytochalasin B. The potential of platelets to migrate in an SDF-1-mediated fashion may redefine the role of platelets in the pathophysiology of vascular inflammation, subsequent atherosclerotic degeneration, and vascular regeneration.
Original language | English (US) |
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Pages (from-to) | 1277-1288 |
Number of pages | 12 |
Journal | Journal of Molecular Medicine |
Volume | 88 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2010 |
Keywords
- Cell migration
- Cell signaling
- Inflammation
- Platelets
- SDF-1
ASJC Scopus subject areas
- Molecular Medicine
- Drug Discovery
- Genetics(clinical)