Physicochemical characterization of the androgen receptor in rat uterine cytosol

C. H. Chang, D. J. Tindall

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this manuscript a number of physicochemical and DNA-binding properties of the androgen receptor from rat uterus were characterized. The dissociation of [3H]testosterone from the receptor followed first order kinetics, with a half-time dissociation rate of approximately 48 h at 0 C. Maximum binding was maintained at pH 7. Ammonium sulfate precipitated the receptor at concentrations between 25-40% saturation. Gel filtration and sucrose gradient analysis revealed a 62Å, 6S binding component (mol wt, 167,000; frictional ratio, 1.71) in both the absence and presence of the protease inhibitor leupeptin. Isoelectric focusing revealed an isoelectric point of 5.9. The receptor bound to DEAE-agarose, heparin-Sepharose, and hydroxylapatite. Increased receptor binding to phosphocellulose and DNA-cellulose was observed after heat treatment at 24 C for 30 min. These results demonstrate that the uterine androgen receptor has many properties similar to those of androgen receptors in male target tissues.

Original languageEnglish (US)
Pages (from-to)1486-1493
Number of pages8
JournalEndocrinology
Volume113
Issue number4
StatePublished - 1983
Externally publishedYes

Fingerprint

Androgen Receptors
Cytosol
Isoelectric Point
Ammonium Sulfate
Isoelectric Focusing
Durapatite
Protease Inhibitors
Sepharose
Uterus
Gel Chromatography
Sucrose
Hot Temperature
DNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Chang, C. H., & Tindall, D. J. (1983). Physicochemical characterization of the androgen receptor in rat uterine cytosol. Endocrinology, 113(4), 1486-1493.

Physicochemical characterization of the androgen receptor in rat uterine cytosol. / Chang, C. H.; Tindall, D. J.

In: Endocrinology, Vol. 113, No. 4, 1983, p. 1486-1493.

Research output: Contribution to journalArticle

Chang, CH & Tindall, DJ 1983, 'Physicochemical characterization of the androgen receptor in rat uterine cytosol', Endocrinology, vol. 113, no. 4, pp. 1486-1493.
Chang, C. H. ; Tindall, D. J. / Physicochemical characterization of the androgen receptor in rat uterine cytosol. In: Endocrinology. 1983 ; Vol. 113, No. 4. pp. 1486-1493.
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