Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Kee Won Lee, Shanfeng Wang, Michael J. Yaszemski, Lichun Lu

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

Original languageEnglish (US)
Pages (from-to)2839-2848
Number of pages10
JournalBiomaterials
Volume29
Issue number19
DOIs
StatePublished - Jul 1 2008

Keywords

  • Hydroxyapatite (HA)
  • Nanocomposite
  • Osteoblast response
  • Poly(propylene fumarate) (PPF)
  • Protein adsorption

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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