Physical mapping of inhibin β-A in domestic cattle

Holly L. Neibergs, Daniel S. Gallagher, Michel Georges, Leslie S. Sargeant, Allan B. Dietz, James E. Womack

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The gene for the β-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalMammalian Genome
Volume4
Issue number6
DOIs
StatePublished - Jun 1 1993

ASJC Scopus subject areas

  • Genetics

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    Neibergs, H. L., Gallagher, D. S., Georges, M., Sargeant, L. S., Dietz, A. B., & Womack, J. E. (1993). Physical mapping of inhibin β-A in domestic cattle. Mammalian Genome, 4(6), 328-332. https://doi.org/10.1007/BF00357092