Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient

Apostolos G. Doukas; Daniel J. McAuliffe; Shun Lee; Vasan Venugopalan; Thomas J. Flotte

doi:10.1016/0301-5629(95)00027-O

Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient

Apostolos G. Doukas, Daniel J. McAuliffe, Shun Lee, Vasan Venugopalan, Thomas J. Flotte

Research output: Contribution to journal › Article

69 Scopus citations

Abstract

We have studied the biological effects of ablation-induced stress waves in vitro. Mouse breast sarcoma cells (EMT-6) were exposed to stress waves that differed only in rise time. Two assays were used to determine cell injury: incorporation of tritiated thymidine (viability assay), and transmission electron microscopy (morphology assay). We present evidence that the rise time of stress waves can significantly modify cell viability and that cell injury correlates better with the stress gradient than peak stress.

Original language	English (US)
Pages (from-to)	961-967
Number of pages	7
Journal	Ultrasound in Medicine and Biology
Volume	21
Issue number	7
DOIs	https://doi.org/10.1016/0301-5629(95)00027-O
State	Published - 1995
Externally published	Yes

Keywords

Ablation
Cell viability
Membrane permeability
Photoacoustics
Shock waves
Stress gradient

ASJC Scopus subject areas

Biophysics
Radiological and Ultrasound Technology
Acoustics and Ultrasonics

Access to Document

10.1016/0301-5629(95)00027-O

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Doukas, A. G., McAuliffe, D. J., Lee, S., Venugopalan, V., & Flotte, T. J. (1995). Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient. Ultrasound in Medicine and Biology, 21(7), 961-967. https://doi.org/10.1016/0301-5629(95)00027-O

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AU - Venugopalan, Vasan

AU - Flotte, Thomas J.

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KW - Membrane permeability

KW - Photoacoustics

KW - Shock waves

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