Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient

Apostolos G. Doukas; Daniel J. McAuliffe; Shun Lee; Vasan Venugopalan; Thomas J. Flotte

doi:10.1016/0301-5629(95)00027-O

Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient

Apostolos G. Doukas, Daniel J. McAuliffe, Shun Lee, Vasan Venugopalan, Thomas J. Flotte

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

We have studied the biological effects of ablation-induced stress waves in vitro. Mouse breast sarcoma cells (EMT-6) were exposed to stress waves that differed only in rise time. Two assays were used to determine cell injury: incorporation of tritiated thymidine (viability assay), and transmission electron microscopy (morphology assay). We present evidence that the rise time of stress waves can significantly modify cell viability and that cell injury correlates better with the stress gradient than peak stress.

Original language	English (US)
Pages (from-to)	961-967
Number of pages	7
Journal	Ultrasound in Medicine and Biology
Volume	21
Issue number	7
DOIs	https://doi.org/10.1016/0301-5629(95)00027-O
State	Published - 1995

Keywords

Ablation
Cell viability
Membrane permeability
Photoacoustics
Shock waves
Stress gradient

ASJC Scopus subject areas

Biophysics
Radiological and Ultrasound Technology
Acoustics and Ultrasonics

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10.1016/0301-5629(95)00027-O

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Cite this

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title = "Physical factors involved in stress-wave-induced cell injury: The effect of stress gradient",

abstract = "We have studied the biological effects of ablation-induced stress waves in vitro. Mouse breast sarcoma cells (EMT-6) were exposed to stress waves that differed only in rise time. Two assays were used to determine cell injury: incorporation of tritiated thymidine (viability assay), and transmission electron microscopy (morphology assay). We present evidence that the rise time of stress waves can significantly modify cell viability and that cell injury correlates better with the stress gradient than peak stress.",

keywords = "Ablation, Cell viability, Membrane permeability, Photoacoustics, Shock waves, Stress gradient",

author = "Doukas, {Apostolos G.} and McAuliffe, {Daniel J.} and Shun Lee and Vasan Venugopalan and Flotte, {Thomas J.}",

note = "Funding Information: Acknowledgements-We thank Peggy Sherwood for the transmission electron microscopy, and Drs. Thomas Deutsch and Reginald Bimgmber for many helpful discussions. This work was supported by the DOD Medical Free Electron Laser under Contract N00014-91-C-0084.",

year = "1995",

doi = "10.1016/0301-5629(95)00027-O",

language = "English (US)",

volume = "21",

pages = "961--967",

journal = "Ultrasound in Medicine and Biology",

issn = "0301-5629",

publisher = "Elsevier USA",

number = "7",

}

TY - JOUR

T1 - Physical factors involved in stress-wave-induced cell injury

T2 - The effect of stress gradient

AU - Doukas, Apostolos G.

AU - McAuliffe, Daniel J.

AU - Lee, Shun

AU - Venugopalan, Vasan

AU - Flotte, Thomas J.

N1 - Funding Information: Acknowledgements-We thank Peggy Sherwood for the transmission electron microscopy, and Drs. Thomas Deutsch and Reginald Bimgmber for many helpful discussions. This work was supported by the DOD Medical Free Electron Laser under Contract N00014-91-C-0084.

PY - 1995

Y1 - 1995

N2 - We have studied the biological effects of ablation-induced stress waves in vitro. Mouse breast sarcoma cells (EMT-6) were exposed to stress waves that differed only in rise time. Two assays were used to determine cell injury: incorporation of tritiated thymidine (viability assay), and transmission electron microscopy (morphology assay). We present evidence that the rise time of stress waves can significantly modify cell viability and that cell injury correlates better with the stress gradient than peak stress.

AB - We have studied the biological effects of ablation-induced stress waves in vitro. Mouse breast sarcoma cells (EMT-6) were exposed to stress waves that differed only in rise time. Two assays were used to determine cell injury: incorporation of tritiated thymidine (viability assay), and transmission electron microscopy (morphology assay). We present evidence that the rise time of stress waves can significantly modify cell viability and that cell injury correlates better with the stress gradient than peak stress.

KW - Ablation

KW - Cell viability

KW - Membrane permeability

KW - Photoacoustics

KW - Shock waves

KW - Stress gradient

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