TY - JOUR
T1 - Physical and enzymatic perturbation of the architecture of the Tunica media destroys its inherent thromboresistance
AU - Komorowicz, Erzsebet
AU - McBane, Robert D.
AU - Fass, David N.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - The media layer of the arterial cryo-cross sections, is defective for vWf-dependent platelet adhesion. Exposure of the same layer by stripping off the most inner portions of the vessel wall results in a highly thrombogenic surface. Stripping or balloon dilation was applied to porcine arteries prior to functional assays. Cryosections of treated or untreated arteries were perfused with porcine blood at 3350 s-1 and platelet deposition was detected by indirect immunofluorescence. Following balloon dilation, vWf-dependent platelet deposition increased; covering 9.08 ± 1.36% of the total media surface area, this value for untreated vessels was 0.88 ± 0.14%. A 10-fold increase was also found in the binding of vWf-coated fluorescent beads to the media. In addition to mechanical procedures, treatment by serine-proteases like trypsin, chymotrypsin and proteinase 3, or by chondroitinase ABC, but not by heparitinase also resulted in a 7-10-fold increase in platelet coverage over the media. Collagen in the media may be complexed with another vessel wall component shielding the vWf-binding sites. Mechanical or biochemical processes unmask these sites, and increase the thrombogenicity of the vessel wall.
AB - The media layer of the arterial cryo-cross sections, is defective for vWf-dependent platelet adhesion. Exposure of the same layer by stripping off the most inner portions of the vessel wall results in a highly thrombogenic surface. Stripping or balloon dilation was applied to porcine arteries prior to functional assays. Cryosections of treated or untreated arteries were perfused with porcine blood at 3350 s-1 and platelet deposition was detected by indirect immunofluorescence. Following balloon dilation, vWf-dependent platelet deposition increased; covering 9.08 ± 1.36% of the total media surface area, this value for untreated vessels was 0.88 ± 0.14%. A 10-fold increase was also found in the binding of vWf-coated fluorescent beads to the media. In addition to mechanical procedures, treatment by serine-proteases like trypsin, chymotrypsin and proteinase 3, or by chondroitinase ABC, but not by heparitinase also resulted in a 7-10-fold increase in platelet coverage over the media. Collagen in the media may be complexed with another vessel wall component shielding the vWf-binding sites. Mechanical or biochemical processes unmask these sites, and increase the thrombogenicity of the vessel wall.
KW - Collagen
KW - Platelets
KW - Proteoglycan
KW - Vascular media
KW - Von Willebrand factor
UR - http://www.scopus.com/inward/record.url?scp=0036845618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036845618&partnerID=8YFLogxK
U2 - 10.1055/s-0037-1613310
DO - 10.1055/s-0037-1613310
M3 - Article
C2 - 12428102
AN - SCOPUS:0036845618
SN - 0340-6245
VL - 88
SP - 827
EP - 833
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 5
ER -