Photodynamic therapy with local photosensitizer delivery inhibits experimental intimal hyperplasia

F. Adili, R. G. Statius van Eps, T. J. Flotte, Thomas J Flotte

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Photodynamic therapy (PDT), the light activation of photosensitizer dyes for the production of free radicals, effectively inhibits experimental intimal hyperplasia with systemic administration of the photosensitizer. The local application of the photosensitizer directly into a vascular lesion to avoid systemic side effects and tightly control dose administration has theoretical appeal. The aim of this study was to quantify and arterial tissue uptake after site-specific photosensitizer delivery and, following PDT, determine its effectiveness at inhibiting intimal hyperplasia. Study Design/Materials and Methods: The rat common carotid artery was balloon-injured, pressurized at 400 mm Hg for 2 minutes with the photosensitizer dye benzoporphyrin-derivative (BPD), and irradiated with 690 nm laser light at a fluence of 100 J/cm 2. Control animals were pressurized with saline only, or received no additional treatment than balloon-injury. Results: Pressurization with BPD resulted in complete penetration of the intima and media and was associated with relatively high tissue, but almost no detectable serum BPD concentrations. No skin photosensitization or other systemic side effects were observed with photosensitizer administration. After 9 days, PDT-treated arteries displayed a significantly lower number of smooth muscle cells in the arterial wall than balloon-injured (P < 0.001) or saline-pressurized arteries (P < 0.0002), and no intimal hyperplasia. At 21 days, IH after PDT was significantly reduced as compared with balloon- injured (P < 0.0004), or saline-pressurized arteries (P < 0.003) with no arterial dilatation. Conclusions: Site-specific delivery of liposomal BPD followed by PDT represents a safe method to treat arteries, and may be effectively used in vivo to inhibit the development of intimal hyperplasia.

Original languageEnglish (US)
Pages (from-to)263-273
Number of pages11
JournalLasers in Surgery and Medicine
Volume23
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Tunica Intima
Photosensitizing Agents
Photochemotherapy
Hyperplasia
Arteries
Coloring Agents
Photosensitivity Disorders
Light
Common Carotid Artery
Free Radicals
Smooth Muscle Myocytes
Blood Vessels
Dilatation
Lasers
Skin
Wounds and Injuries
Serum

ASJC Scopus subject areas

  • Surgery

Cite this

Photodynamic therapy with local photosensitizer delivery inhibits experimental intimal hyperplasia. / Adili, F.; Statius van Eps, R. G.; Flotte, T. J.; Flotte, Thomas J.

In: Lasers in Surgery and Medicine, Vol. 23, No. 5, 1998, p. 263-273.

Research output: Contribution to journalArticle

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