TY - JOUR
T1 - Photo-crosslinked poly(ε-caprolactone fumarate) networks for guided peripheral nerve regeneration
T2 - Material properties and preliminary biological evaluations
AU - Wang, Shanfeng
AU - Yaszemski, Michael J.
AU - Knight, Andrew M.
AU - Gruetzmacher, James A.
AU - Windebank, Anthony J.
AU - Lu, Lichun
N1 - Funding Information:
This work was performed at Mayo Clinic with support from the Mayo Foundation and NIH (R01 AR45871 and R01 EB003060). The authors thank Jarred J. Nesbitt and Dr. Huan Wang at Mayo Clinic for technical assistance in SPL201 cell culture and animal surgery, respectively. We also thank Drs. Jack L. Lewis and Narendra K. Simha at the University of Minnesota for allowing the use of micro-indenter.
PY - 2009/6
Y1 - 2009/6
N2 - In an effort to achieve suitable biomaterials for peripheral nerve regeneration, we present a material design strategy of combining a crystallite-based physical network and a crosslink-based chemical network. Biodegradable polymer disks and conduits have been fabricated by photo-crosslinking three poly(ε-caprolactone fumarate)s (PCLF530, PCLF1250, and PCLF2000), which were synthesized from the precursor poly(ε-caprolactone) (PCL) diols with nominal molecular weights of 530, 1250, and 2000 g mol-1, respectively. Thermal properties such as glass transition temperature (Tg), melting temperature (Tm), and crystallinity of photo-crosslinked PCLFs were examined and correlated with their rheological and mechanical properties. Furthermore, in vitro degradation of uncrosslinked and crosslinked PCLFs in PBS crosslinked PCLFs in 1 N NaOH aqueous solution at 37 °C was studied. In vitro cytocompatibility, attachment, and proliferation of Schwann cell precursor line SPL201 cells on three PCLF networks were investigated. Crosslinked PCLF2000 with the highest crystallinity and mechanical properties was found to best support cell attachment and proliferation. Using a new photo-crosslinking method, single-lumen crosslinked PCLF nerve conduits without defects were fabricated in a glass mold. Crosslinked PCLF2000 nerve conduits were selected for evaluation in a 1 cm gap rat sciatic nerve model. Histological evaluation demonstrated that the material was biocompatible with sufficient strength to hold sutures in place after 6 and 17 weeks of implantation. Nerve cable with myelinated axons was found in the crosslinked PCLF2000 nerve conduit.
AB - In an effort to achieve suitable biomaterials for peripheral nerve regeneration, we present a material design strategy of combining a crystallite-based physical network and a crosslink-based chemical network. Biodegradable polymer disks and conduits have been fabricated by photo-crosslinking three poly(ε-caprolactone fumarate)s (PCLF530, PCLF1250, and PCLF2000), which were synthesized from the precursor poly(ε-caprolactone) (PCL) diols with nominal molecular weights of 530, 1250, and 2000 g mol-1, respectively. Thermal properties such as glass transition temperature (Tg), melting temperature (Tm), and crystallinity of photo-crosslinked PCLFs were examined and correlated with their rheological and mechanical properties. Furthermore, in vitro degradation of uncrosslinked and crosslinked PCLFs in PBS crosslinked PCLFs in 1 N NaOH aqueous solution at 37 °C was studied. In vitro cytocompatibility, attachment, and proliferation of Schwann cell precursor line SPL201 cells on three PCLF networks were investigated. Crosslinked PCLF2000 with the highest crystallinity and mechanical properties was found to best support cell attachment and proliferation. Using a new photo-crosslinking method, single-lumen crosslinked PCLF nerve conduits without defects were fabricated in a glass mold. Crosslinked PCLF2000 nerve conduits were selected for evaluation in a 1 cm gap rat sciatic nerve model. Histological evaluation demonstrated that the material was biocompatible with sufficient strength to hold sutures in place after 6 and 17 weeks of implantation. Nerve cable with myelinated axons was found in the crosslinked PCLF2000 nerve conduit.
KW - Cell responses
KW - Peripheral nerve regeneration
KW - Photo-crosslinking
KW - Poly(ε-caprolactone fumarate)
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U2 - 10.1016/j.actbio.2008.12.015
DO - 10.1016/j.actbio.2008.12.015
M3 - Article
C2 - 19171506
AN - SCOPUS:65349149957
SN - 1742-7061
VL - 5
SP - 1531
EP - 1542
JO - Acta Biomaterialia
JF - Acta Biomaterialia
IS - 5
ER -