Phosphorylation-regulated CI- channel in CHO cells stably expressing the cystic fibrosis gene

Joseph A. Tabcharani, Xiu Bao Chang, John R. Riordan, John W. Hanrahan

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Abstract

A cyclic AMP-stimulated choride conductance appears when the cystic fibres is gene is expressed in non-epithelial cells by infection with recombinant viruses1,2. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia2-4, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibres is gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.

Original languageEnglish (US)
Pages (from-to)628-631
Number of pages4
JournalNature
Volume352
Issue number6336
DOIs
StatePublished - Jan 1 1991

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    Tabcharani, J. A., Chang, X. B., Riordan, J. R., & Hanrahan, J. W. (1991). Phosphorylation-regulated CI- channel in CHO cells stably expressing the cystic fibrosis gene. Nature, 352(6336), 628-631. https://doi.org/10.1038/352628a0