Phosphorylation of glucose analog 3-0-methyl-D-glucose by rat heart

S. John Gatley, James E. Holden, James R. Halama, Timothy R DeGrado, Dana R. Bernstein, Chin K. Ng

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

When isolated rat hearts were perfused with medium containing 3-0-methyl-D-glucose (3MG) a compound with the chromatographic behavior expected for 3MG-6-phosphate was formed. This assignment is supported by the action of alkaline phosphatase and by production of an identical peak on incubation of 3MG with yeast hexokinase in a buffer containing adenosine triphosphate and Mg++ ions. The clearance of labeled 3MG from isolated perfused rat hearts was biphasic and eventually total. Since sugar phosphates do not cross cellular membranes, this behavior is consistent with both formation and dephosphorylation of 3MG-6-phosphate, with dephosphorylation rate-limiting the slow phase of the clearance. Phosphatase activity was also indicated by a decrease in 3MG-6-phosphate when hearts were not chilled and homogenized immediately after perfusion. The common assumption that 3MG is not metabolized after transport into cells is shown by these experiments to be erroneous.

Original languageEnglish (US)
Pages (from-to)1008-1014
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume119
Issue number3
DOIs
StatePublished - Mar 30 1984
Externally publishedYes

Fingerprint

Phosphorylation
Rats
Glucose
Glucose-6-Phosphate
Sugar Phosphates
Hexokinase
Phosphoric Monoester Hydrolases
Yeast
Alkaline Phosphatase
Buffers
Perfusion
Adenosine Triphosphate
Yeasts
Ions
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Phosphorylation of glucose analog 3-0-methyl-D-glucose by rat heart. / Gatley, S. John; Holden, James E.; Halama, James R.; DeGrado, Timothy R; Bernstein, Dana R.; Ng, Chin K.

In: Biochemical and Biophysical Research Communications, Vol. 119, No. 3, 30.03.1984, p. 1008-1014.

Research output: Contribution to journalArticle

Gatley, S. John ; Holden, James E. ; Halama, James R. ; DeGrado, Timothy R ; Bernstein, Dana R. ; Ng, Chin K. / Phosphorylation of glucose analog 3-0-methyl-D-glucose by rat heart. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 119, No. 3. pp. 1008-1014.
@article{e5bd23301b7d416f84b8c0be0d71b3a3,
title = "Phosphorylation of glucose analog 3-0-methyl-D-glucose by rat heart",
abstract = "When isolated rat hearts were perfused with medium containing 3-0-methyl-D-glucose (3MG) a compound with the chromatographic behavior expected for 3MG-6-phosphate was formed. This assignment is supported by the action of alkaline phosphatase and by production of an identical peak on incubation of 3MG with yeast hexokinase in a buffer containing adenosine triphosphate and Mg++ ions. The clearance of labeled 3MG from isolated perfused rat hearts was biphasic and eventually total. Since sugar phosphates do not cross cellular membranes, this behavior is consistent with both formation and dephosphorylation of 3MG-6-phosphate, with dephosphorylation rate-limiting the slow phase of the clearance. Phosphatase activity was also indicated by a decrease in 3MG-6-phosphate when hearts were not chilled and homogenized immediately after perfusion. The common assumption that 3MG is not metabolized after transport into cells is shown by these experiments to be erroneous.",
author = "Gatley, {S. John} and Holden, {James E.} and Halama, {James R.} and DeGrado, {Timothy R} and Bernstein, {Dana R.} and Ng, {Chin K.}",
year = "1984",
month = "3",
day = "30",
doi = "10.1016/0006-291X(84)90874-X",
language = "English (US)",
volume = "119",
pages = "1008--1014",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Phosphorylation of glucose analog 3-0-methyl-D-glucose by rat heart

AU - Gatley, S. John

AU - Holden, James E.

AU - Halama, James R.

AU - DeGrado, Timothy R

AU - Bernstein, Dana R.

AU - Ng, Chin K.

PY - 1984/3/30

Y1 - 1984/3/30

N2 - When isolated rat hearts were perfused with medium containing 3-0-methyl-D-glucose (3MG) a compound with the chromatographic behavior expected for 3MG-6-phosphate was formed. This assignment is supported by the action of alkaline phosphatase and by production of an identical peak on incubation of 3MG with yeast hexokinase in a buffer containing adenosine triphosphate and Mg++ ions. The clearance of labeled 3MG from isolated perfused rat hearts was biphasic and eventually total. Since sugar phosphates do not cross cellular membranes, this behavior is consistent with both formation and dephosphorylation of 3MG-6-phosphate, with dephosphorylation rate-limiting the slow phase of the clearance. Phosphatase activity was also indicated by a decrease in 3MG-6-phosphate when hearts were not chilled and homogenized immediately after perfusion. The common assumption that 3MG is not metabolized after transport into cells is shown by these experiments to be erroneous.

AB - When isolated rat hearts were perfused with medium containing 3-0-methyl-D-glucose (3MG) a compound with the chromatographic behavior expected for 3MG-6-phosphate was formed. This assignment is supported by the action of alkaline phosphatase and by production of an identical peak on incubation of 3MG with yeast hexokinase in a buffer containing adenosine triphosphate and Mg++ ions. The clearance of labeled 3MG from isolated perfused rat hearts was biphasic and eventually total. Since sugar phosphates do not cross cellular membranes, this behavior is consistent with both formation and dephosphorylation of 3MG-6-phosphate, with dephosphorylation rate-limiting the slow phase of the clearance. Phosphatase activity was also indicated by a decrease in 3MG-6-phosphate when hearts were not chilled and homogenized immediately after perfusion. The common assumption that 3MG is not metabolized after transport into cells is shown by these experiments to be erroneous.

UR - http://www.scopus.com/inward/record.url?scp=0021234488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021234488&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(84)90874-X

DO - 10.1016/0006-291X(84)90874-X

M3 - Article

VL - 119

SP - 1008

EP - 1014

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -