Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-κB activation

Hu Zeng, Lie Di, Guoping Fu, Yuhong Chen, Xiang Gao, Langlai Xu, Xin Lin, Renren Wen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Bcl10 (B-cell lymphoma 10) is an adaptor protein comprised of an N-tenninal caspase recruitment domain and a C-terminal serine/threonine-rich domain. Bcl10 plays a critical role in antigen receptor-mediated NF-κB activation and lymphocyte development and functions. Our current study has discovered that T-cell activation induced monophosphorylation and biphosphorylation of Bcl10 and has identified S138 within Bcl10 as one of the T-cell receptor-induced phosphorylation sites. Alteration of S138 to an alanine residue impaired T-cell activation-induced ubiquitination and subsequent degradation of Bcl10, ultimately resulting in prolongation of TCR-mediated NF-κB activation and enhancement of interleukin-2 production. Taken together, our findings demonstrate that phosphorylation of Bcl10 at S138 down-regulates Bcl10 protein levels and thus negatively regulates T-cell receptor-mediated NF-κB activation.

Original languageEnglish (US)
Pages (from-to)5235-5245
Number of pages11
JournalMolecular and Cellular Biology
Volume27
Issue number14
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

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B-Cell Lymphoma
T-Cell Antigen Receptor
Phosphorylation
T-Lymphocytes
Antigen Receptors
Ubiquitination
Threonine
Lymphocyte Activation
Alanine
Serine
Interleukin-2
Proteins
Down-Regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-κB activation. / Zeng, Hu; Di, Lie; Fu, Guoping; Chen, Yuhong; Gao, Xiang; Xu, Langlai; Lin, Xin; Wen, Renren.

In: Molecular and Cellular Biology, Vol. 27, No. 14, 01.07.2007, p. 5235-5245.

Research output: Contribution to journalArticle

Zeng, Hu ; Di, Lie ; Fu, Guoping ; Chen, Yuhong ; Gao, Xiang ; Xu, Langlai ; Lin, Xin ; Wen, Renren. / Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-κB activation. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 14. pp. 5235-5245.
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AU - Xu, Langlai

AU - Lin, Xin

AU - Wen, Renren

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