Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu

Valerie E. Martindale, Jeffrey L Salisbury

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Centrin, a calcium-sensitive contractile phosphoprotein, is the major component of the striated flagellar roots of the flagellate green alga, Tetraselmis striata. Flagellar roots contract in response to elevated calcium levels. Data presented here indicate that the level of centrin phosphorylation is rapidly responsive to changes in the cell's external environment. Centrin is dephosphorylated in response to elevated calcium or to heat shock. An increase in centrin phosphorylation accompanies pH shock or ethanol treatment. These changes are compared with flagellar excision, flagellar root contraction, and protein synthesis under the same treatments. We conclude that under certain conditions phosphorylation/dephosphorylation of centrin and extension/contraction of the flagellar root are uncoupled. Possible role(s) of centrin phosphorylation independent of force generation in the flagellar root are discussed.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalJournal of Cell Science
Volume96
Issue number3
StatePublished - Jul 1990

Fingerprint

Sulfamethoxazole Drug Combination Trimethoprim
Phosphorylation
Calcium
Shock
Chlorophyta
Phosphoproteins
Contracts
Ethanol
Hot Temperature
Proteins

Keywords

  • Centrin
  • Flagellar root
  • Phosphorylation
  • Tetraselmis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu. / Martindale, Valerie E.; Salisbury, Jeffrey L.

In: Journal of Cell Science, Vol. 96, No. 3, 07.1990, p. 395-402.

Research output: Contribution to journalArticle

@article{658c233a482249b4b04415cde70f897b,
title = "Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu",
abstract = "Centrin, a calcium-sensitive contractile phosphoprotein, is the major component of the striated flagellar roots of the flagellate green alga, Tetraselmis striata. Flagellar roots contract in response to elevated calcium levels. Data presented here indicate that the level of centrin phosphorylation is rapidly responsive to changes in the cell's external environment. Centrin is dephosphorylated in response to elevated calcium or to heat shock. An increase in centrin phosphorylation accompanies pH shock or ethanol treatment. These changes are compared with flagellar excision, flagellar root contraction, and protein synthesis under the same treatments. We conclude that under certain conditions phosphorylation/dephosphorylation of centrin and extension/contraction of the flagellar root are uncoupled. Possible role(s) of centrin phosphorylation independent of force generation in the flagellar root are discussed.",
keywords = "Centrin, Flagellar root, Phosphorylation, Tetraselmis",
author = "Martindale, {Valerie E.} and Salisbury, {Jeffrey L}",
year = "1990",
month = "7",
language = "English (US)",
volume = "96",
pages = "395--402",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "3",

}

TY - JOUR

T1 - Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu

AU - Martindale, Valerie E.

AU - Salisbury, Jeffrey L

PY - 1990/7

Y1 - 1990/7

N2 - Centrin, a calcium-sensitive contractile phosphoprotein, is the major component of the striated flagellar roots of the flagellate green alga, Tetraselmis striata. Flagellar roots contract in response to elevated calcium levels. Data presented here indicate that the level of centrin phosphorylation is rapidly responsive to changes in the cell's external environment. Centrin is dephosphorylated in response to elevated calcium or to heat shock. An increase in centrin phosphorylation accompanies pH shock or ethanol treatment. These changes are compared with flagellar excision, flagellar root contraction, and protein synthesis under the same treatments. We conclude that under certain conditions phosphorylation/dephosphorylation of centrin and extension/contraction of the flagellar root are uncoupled. Possible role(s) of centrin phosphorylation independent of force generation in the flagellar root are discussed.

AB - Centrin, a calcium-sensitive contractile phosphoprotein, is the major component of the striated flagellar roots of the flagellate green alga, Tetraselmis striata. Flagellar roots contract in response to elevated calcium levels. Data presented here indicate that the level of centrin phosphorylation is rapidly responsive to changes in the cell's external environment. Centrin is dephosphorylated in response to elevated calcium or to heat shock. An increase in centrin phosphorylation accompanies pH shock or ethanol treatment. These changes are compared with flagellar excision, flagellar root contraction, and protein synthesis under the same treatments. We conclude that under certain conditions phosphorylation/dephosphorylation of centrin and extension/contraction of the flagellar root are uncoupled. Possible role(s) of centrin phosphorylation independent of force generation in the flagellar root are discussed.

KW - Centrin

KW - Flagellar root

KW - Phosphorylation

KW - Tetraselmis

UR - http://www.scopus.com/inward/record.url?scp=0025299171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025299171&partnerID=8YFLogxK

M3 - Article

C2 - 2229193

AN - SCOPUS:0025299171

VL - 96

SP - 395

EP - 402

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 3

ER -