Phosphorylation-mediated 14-3-3 protein binding regulates the function of the rho-specific guanine nucleotide exchange factor (RhoGEF) syx

Siu P. Ngok, Rory Geyer, Antonis Kourtidis, Peter Storz, Panagiotis Z Anastasiadis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Syx is a Rho-specific guanine nucleotide exchange factor (GEF) that localizes at cell-cell junctions and promotes junction stability by activating RhoA and the downstream effector Diaphanous homolog 1 (Dia1). Previously, we identified several molecules, including 14-3-3 proteins, as Syx-interacting partners. In the present study, we show that 14-3-3 isoforms interact with Syx at both its N- and C-terminal regions in a phosphorylation- dependent manner. We identify the protein kinase D-mediated phosphorylation of serine 92 on Syx, and additional phosphorylation at serine 938, as critical sites for 14-3-3 association. Our data indicate that the binding of 14-3-3 proteins inhibits the GEF activity of Syx. Furthermore, we show that phosphorylation-deficient, 14-3-3-uncoupled Syx exhibits increased junctional targeting and increased GEF activity, resulting in the strengthening of the circumferential junctional actin ring in Madin-Darby canine kidney cells. These findings reveal a novel means of regulating junctional Syx localization and function by phosphorylation-induced 14-3-3 binding and further support the importance of Syx function in maintaining stable cell-cell contacts.

Original languageEnglish (US)
Pages (from-to)6640-6650
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number9
DOIs
StatePublished - Mar 1 2013

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Rho Guanine Nucleotide Exchange Factors
14-3-3 Proteins
Guanine Nucleotide Exchange Factors
Phosphorylation
Protein Binding
Serine
Madin Darby Canine Kidney Cells
Intercellular Junctions
Actins
Protein Isoforms
Association reactions
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Phosphorylation-mediated 14-3-3 protein binding regulates the function of the rho-specific guanine nucleotide exchange factor (RhoGEF) syx. / Ngok, Siu P.; Geyer, Rory; Kourtidis, Antonis; Storz, Peter; Anastasiadis, Panagiotis Z.

In: Journal of Biological Chemistry, Vol. 288, No. 9, 01.03.2013, p. 6640-6650.

Research output: Contribution to journalArticle

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