Phosphorylation events associated with cyclic nucleotide-dependent inhibition of smooth muscle contraction

David A Woodrum, Colleen M. Brophy, Christopher J. Wingard, Arthur Beall, Howard Rasmussen

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Activation of cyclic nucleotide-dependent signaling pathways leads to relaxation of bovine carotid artery smooth muscle contractions and is associated with increased phosphorylation of the small heat shock-related protein (HSP20). Previous reports have shown that human umbilical artery smooth muscle is uniquely resistant to cyclic nucleotide-dependent relaxation, and HSP20 is not phosphorylated. In this investigation, we determined the phosphorylation events associated with cyclic nucleotide- dependent inhibition of smooth muscle contraction. In carotid artery, activation of cyclic nucleotide-dependent signaling pathways inhibited contractile responses to serotonin but did not inhibit myosin light chain phosphorylation or oxygen consumption. The inhibition of contraction was associated with increases in HSP20 phosphorylation. In umbilical artery, activation of cyclic nucleotide-dependent signaling pathways did not inhibit serotonin-induced contraction or myosin light chain phosphorylation. The lack of contractile inhibition in umbilical artery was not associated with significant increases in HSP20 phosphorylation. In conclusion, cyclic nucleotide-dependent contractile inhibition is independent of the inhibition of myosin light chain phosphorylation or oxygen consumption but does correlate with increased HSP20 phosphorylation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume277
Issue number3 46-3
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Cyclic Nucleotides
Muscle Contraction
Smooth Muscle
Phosphorylation
Myosin Light Chains
Umbilical Arteries
Carotid Arteries
Oxygen Consumption
Serotonin
Small Heat-Shock Proteins

Keywords

  • Heat shock proteins
  • Myosin light chains
  • Oxygen consumption

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Phosphorylation events associated with cyclic nucleotide-dependent inhibition of smooth muscle contraction. / Woodrum, David A; Brophy, Colleen M.; Wingard, Christopher J.; Beall, Arthur; Rasmussen, Howard.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 277, No. 3 46-3, 09.1999.

Research output: Contribution to journalArticle

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