Abstract
The new approaches for systematic phosphoprotein analysis by mass spectrometry (MS) were described. The advancements included enrichment and identification of phosphoserine/phosphothreonine proteins from cells, identification of protein phosphorylation sites by capillary LC-MS/MS and evaluation of LC-MS/MS neutral loss scan on a QTOF instrument for phosphopeptide detection and sequencing. 7 human phosphoproteins were identified using a novel mass spectrometry based proteomics strategy. To increase the selectivity of data dependent MS/MS a novel precursor ion discovery tool was evaluated for real time phosphopeptide detection duting LC-MS/MS.
| Original language | English (US) |
|---|---|
| Pages | 89-90 |
| Number of pages | 2 |
| State | Published - 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy