Phosphoproteomics: New approaches for systematic phosphoprotein analysis by mass spectrometry

Allan Stensballe, Mads Grønborg, Akhilesh Pandey, Ole Nørregaard Jensen

Research output: Contribution to conferencePaper

Abstract

The new approaches for systematic phosphoprotein analysis by mass spectrometry (MS) were described. The advancements included enrichment and identification of phosphoserine/phosphothreonine proteins from cells, identification of protein phosphorylation sites by capillary LC-MS/MS and evaluation of LC-MS/MS neutral loss scan on a QTOF instrument for phosphopeptide detection and sequencing. 7 human phosphoproteins were identified using a novel mass spectrometry based proteomics strategy. To increase the selectivity of data dependent MS/MS a novel precursor ion discovery tool was evaluated for real time phosphopeptide detection duting LC-MS/MS.

Original languageEnglish (US)
Pages89-90
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Stensballe, A., Grønborg, M., Pandey, A., & Jensen, O. N. (2002). Phosphoproteomics: New approaches for systematic phosphoprotein analysis by mass spectrometry. 89-90. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.