TY - JOUR
T1 - Phosphoproteomics.
AU - Zhong, Jun
AU - Molina, Henrik
AU - Pandey, Akhilesh
PY - 2007/11
Y1 - 2007/11
N2 - Protein phosphorylation is one of the most important mechanisms of regulating protein function in cells, and it plays an important role in controlling diverse biological processes, including cellular proliferation, migration, and metabolism. The term "phosphoproteome" refers to the complement of proteins that undergoes phosphorylation, the extent of their phosphorylation status at the level of individual residues, as well as the dynamics of the phosphorylation events in response to various stimuli. This unit provides methods for enrichment of phosphorylated proteins and peptides using anti-phosphotyrosine antibodies or titanium dioxide, respectively. Support protocols are provided for two detergent-free cell lysis methods, fractionation of proteins prior to enrichment, and use of stable isotope labeling by amino acids in cell culture (SILAC) method for studying dynamics of phosphorylation events. (c) 2007 by John Wiley & Sons, Inc.
AB - Protein phosphorylation is one of the most important mechanisms of regulating protein function in cells, and it plays an important role in controlling diverse biological processes, including cellular proliferation, migration, and metabolism. The term "phosphoproteome" refers to the complement of proteins that undergoes phosphorylation, the extent of their phosphorylation status at the level of individual residues, as well as the dynamics of the phosphorylation events in response to various stimuli. This unit provides methods for enrichment of phosphorylated proteins and peptides using anti-phosphotyrosine antibodies or titanium dioxide, respectively. Support protocols are provided for two detergent-free cell lysis methods, fractionation of proteins prior to enrichment, and use of stable isotope labeling by amino acids in cell culture (SILAC) method for studying dynamics of phosphorylation events. (c) 2007 by John Wiley & Sons, Inc.
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UR - http://www.scopus.com/inward/citedby.url?scp=48849093914&partnerID=8YFLogxK
M3 - Article
C2 - 18429323
AN - SCOPUS:48849093914
SN - 1934-3655
VL - Chapter 24
SP - Unit 24.4
JO - Current protocols in protein science / editorial board, John E. Coligan ... [et al.]
JF - Current protocols in protein science / editorial board, John E. Coligan ... [et al.]
ER -