Phosphoproteome of Cryptococcus neoformans

Lakshmi Dhevi N. Selvan, Santosh Renuse, Jyothi Embekkat Kaviyil, Jyoti Sharma, Sneha M. Pinto, Soujanya D. Yelamanchi, Vinuth N. Puttamallesh, Raju Ravikumar, Akhilesh Pandey, T. S.Keshava Prasad, H. C. Harsha

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cryptococcus neoformans is an encapsulated pathogenic yeast, which causes life threatening meningitis in immunocompromised individuals. C. neoformans var. grubii is the most prevalent and virulent form among the two varieties of C. neoformans - C. neoformans var. grubii and C. neoformans var. neoformans. The virulence of C. neoformans is mainly conferred by its capsule and melanin. cAMP dependent PKA-induced phosphorylation events are reported to be associated with the expression of these virulence traits, which highlights the importance of phosphoproteins in virulence and infection. Therefore, we performed global profiling of phosphoproteome of C. neoformans to enable a better understanding of molecular regulation of its virulence and pathogenesis. High resolution mass spectrometry of TiO2 enriched phosphopeptides from C. neoformans var. grubii grown in culture led to the identification of 1089 phosphopeptides derived from 648 proteins including about 45 kinases. Motif enrichment analysis revealed that most CDK family substrates were found to be phosphorylated. This indicates that cyclin-dependent kinases were among the active kinases in the pathogen in culture. These studies provide a framework for understanding virulence mechanisms in the context of signalling pathways in pathogenic yeast.This article is part of a Special Issue entitled: Trends in Microbial Proteomics. Biological significance: C. neoformans is a pathogenic yeast responsible for cryptococcal meningitis. Melanin and polysaccharide capsule have been established as some of the key virulence factors that play a major role in the pathogenesis of C. neoformans. Recent studies have shown the role of kinase mediated signalling pathways in governing biosynthesis of these virulence factors. This study revealed 1540 phosphorylation sites in 648 proteins providing a comprehensive view of phosphoproteins in C. neoformans. This should serve as a useful resource to explore activated signalling pathways in C. neoformans and their association with its virulence and pathogenesis.

Original languageEnglish (US)
Pages (from-to)287-295
Number of pages9
JournalJournal of Proteomics
Volume97
DOIs
StatePublished - Jan 31 2014
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Yeast
Phosphopeptides
Phosphorylation
Phosphotransferases
Phosphoproteins
Melanins
Virulence Factors
Capsules
Virulence
Cyclin-Dependent Kinases
Biosynthesis
Pathogens
Mass spectrometry
Polysaccharides
Proteins
Association reactions
Yeasts
Substrates
Cryptococcal Meningitis

Keywords

  • Fungal infection
  • Metal oxide affinity chromatography
  • Stress response

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Selvan, L. D. N., Renuse, S., Kaviyil, J. E., Sharma, J., Pinto, S. M., Yelamanchi, S. D., ... Harsha, H. C. (2014). Phosphoproteome of Cryptococcus neoformans. Journal of Proteomics, 97, 287-295. https://doi.org/10.1016/j.jprot.2013.06.029

Phosphoproteome of Cryptococcus neoformans. / Selvan, Lakshmi Dhevi N.; Renuse, Santosh; Kaviyil, Jyothi Embekkat; Sharma, Jyoti; Pinto, Sneha M.; Yelamanchi, Soujanya D.; Puttamallesh, Vinuth N.; Ravikumar, Raju; Pandey, Akhilesh; Prasad, T. S.Keshava; Harsha, H. C.

In: Journal of Proteomics, Vol. 97, 31.01.2014, p. 287-295.

Research output: Contribution to journalArticle

Selvan, LDN, Renuse, S, Kaviyil, JE, Sharma, J, Pinto, SM, Yelamanchi, SD, Puttamallesh, VN, Ravikumar, R, Pandey, A, Prasad, TSK & Harsha, HC 2014, 'Phosphoproteome of Cryptococcus neoformans', Journal of Proteomics, vol. 97, pp. 287-295. https://doi.org/10.1016/j.jprot.2013.06.029
Selvan LDN, Renuse S, Kaviyil JE, Sharma J, Pinto SM, Yelamanchi SD et al. Phosphoproteome of Cryptococcus neoformans. Journal of Proteomics. 2014 Jan 31;97:287-295. https://doi.org/10.1016/j.jprot.2013.06.029
Selvan, Lakshmi Dhevi N. ; Renuse, Santosh ; Kaviyil, Jyothi Embekkat ; Sharma, Jyoti ; Pinto, Sneha M. ; Yelamanchi, Soujanya D. ; Puttamallesh, Vinuth N. ; Ravikumar, Raju ; Pandey, Akhilesh ; Prasad, T. S.Keshava ; Harsha, H. C. / Phosphoproteome of Cryptococcus neoformans. In: Journal of Proteomics. 2014 ; Vol. 97. pp. 287-295.
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