Phosphatidylinositol phosphate kinase type Iγ directly associates with and regulates Shp-1 tyrosine phosphatase

Shawn F. Bairstow, Kun Ling, Richard A. Anderson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Tyrosine phosphorylation plays a critical role in many regulatory aspects of cellular signaling, and dephosphorylation of phosphotyrosine residues is crucial for termination of signals initiated by tyrosine kinases. Previous work has shown that the tyrosine kinase Src phosphorylates Tyr 644 on phosphatidylinositol phosphate kinase type I (PIPKI) γ661 in a focal adhesion kinase-dependent manner. Phosphorylation of this residue is essential for high affinity binding of PIPKIγ661 to the focal adhesion protein talin and for targeting of PIPKIγ661 to focal adhesions. A yeast two-hybrid screen performed with the C-terminal 178-amino acid tail of PIPKIγ661 identified an interaction with the phosphatase domain of the tyrosine phosphatase Shp-1. The interaction between PIPKIγ661 and Shp-1 was confirmed via co-immunoprecipitation from HEK293 cell lysates. In addition, Src-phosphorylated PIPKIγ661 is a substrate for Shp-1, and Shp-1 modulates both the association between PIPKIγ661 and talin and the targeting of PIPKIγ661 to focal adhesions in mammalian cells. Finally, we showed that Shp-1 phosphatase activity is inhibited by the product of PIPKIγ661, phosphatidylinositol 4,5-bisphosphate, in vitro. These combined results suggest a model in which the reciprocal actions of Src tyrosine kinase and Shp-1 tyrosine phosphatase dynamically regulate the association between PIPKIγ661 and talin.

Original languageEnglish (US)
Pages (from-to)23884-23891
Number of pages8
JournalJournal of Biological Chemistry
Volume280
Issue number25
DOIs
StatePublished - Jun 24 2005
Externally publishedYes

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Phosphatidylinositol Phosphates
Talin
Phosphoric Monoester Hydrolases
Focal Adhesions
Tyrosine
Phosphotransferases
Phosphorylation
Adhesion
src-Family Kinases
Association reactions
Cell signaling
Focal Adhesion Protein-Tyrosine Kinases
Phosphotyrosine
HEK293 Cells
Protein Transport
Phosphatidylinositols
Immunoprecipitation
Yeast
Protein-Tyrosine Kinases
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphatidylinositol phosphate kinase type Iγ directly associates with and regulates Shp-1 tyrosine phosphatase. / Bairstow, Shawn F.; Ling, Kun; Anderson, Richard A.

In: Journal of Biological Chemistry, Vol. 280, No. 25, 24.06.2005, p. 23884-23891.

Research output: Contribution to journalArticle

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