Abstract
Herein, we provide evidence that in chicken smooth muscle, G-protein stimulation by a Rho-kinase pathway leads to an increase in myosin light chain phosphorylation. Additionally, G-protein stimulation did not increase MYPT1 phosphorylation at Thr695 or Thr850, and CPI-17, was not expressed in chicken smooth muscle. However, PHI-1 was present in chicken smooth muscle tissues. Both agonist and GTPγS stimulation result in an increase in PHI-1 phosphorylation, which is inhibited by inhibitors to both Rho-kinase (Y-27632) and (PKC) GF109203x. These data suggest that PHI-1 may act as a CPI-17 analog in chicken smooth muscle and inhibit myosin phosphatase activity during G-protein stimulation to produce Ca2+ sensitization.
Original language | English (US) |
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Pages (from-to) | 4271-4277 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 579 |
Issue number | 20 |
DOIs | |
State | Published - Aug 15 2005 |
Keywords
- CPI-17
- Integrin-linked kinase
- MYPT1
- Myosin light chain phosphatase
- PHI-1
- Zip kinase
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology