Phenotypic characterization of the human mast-cell line HMC-1

G. Nilsson, T. Blom, M. Kusche-Gullberg, L. Kjellen, J. H. Butterfield, C. Sundstrom, K. Nilsson, L. Hellman

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Abstract

The cell line HMC-1, derived from a patient with mast cell leukaemia, is the only established cell line exhibiting a phenotype similar to that of human mast cells. This paper reports on a detailed characterization of the expression of a panel of markers for various types of immature and mature haemotopoietic cells in the HMC-1. We also studied the potential of HMC-1 to differentiate upon treatment with conditioned media from the human T-cell line Mo, retinoic acid or DMSO. HMC-1 was found to express several mast cell- related markers. A high expression of Kit, the receptor for stem-cell factor, was detected. The majority of the cells were stained with a MoAb against the mast cell-specific serine protease tryptase. Of particular interest was the finding that β-tryptase mRNA, but not α-tryptase mRNA, was expressed in HMC-1. Using enzyme-histochemistry we were able to show that the β-tryptase was enzymatically active, indicating that tryptase can form active homotetramers. Both heparin and chondroitin sulfate were found to be present in approximately equal amounts. HMC-1 lacked surface expression of the high- affinity IgE receptor, which was confirmed by the absence of mRNA of the α- and β-chains of the IgE-receptor complex. However, a strong expression of the γ-chain of the IgE-receptor complex was detected. A positive staining of the monocyte/macrophage marker CD68 was obtained, as well as a strong hybridization signal for the eosinophilic/basophilic-related differentiation marker the Charcot-Leyden crystal. Treatment of HMC-1 with conditioned media from the human T-cell line Mo, retinoic acid or DMSO induced only moderate changes in the surface or intracellular expression of the studied markers. The agents tested neither induced any of the monocyte/granulocyte markers examined, nor expression of the FcεRIα-chain.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalScandinavian Journal of Immunology
Volume39
Issue number5
DOIs
StatePublished - 1994

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Tryptases
Mast Cells
IgE Receptors
Cell Line
Conditioned Culture Medium
Dimethyl Sulfoxide
Tretinoin
Messenger RNA
Monocytes
Mast-Cell Leukemia
Proto-Oncogene Proteins c-kit
T-Lymphocytes
Chondroitin Sulfates
Differentiation Antigens
Serine Proteases
Granulocytes
Heparin
Macrophages
Staining and Labeling
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Nilsson, G., Blom, T., Kusche-Gullberg, M., Kjellen, L., Butterfield, J. H., Sundstrom, C., ... Hellman, L. (1994). Phenotypic characterization of the human mast-cell line HMC-1. Scandinavian Journal of Immunology, 39(5), 489-498. https://doi.org/10.1111/j.1365-3083.1994.tb03404.x

Phenotypic characterization of the human mast-cell line HMC-1. / Nilsson, G.; Blom, T.; Kusche-Gullberg, M.; Kjellen, L.; Butterfield, J. H.; Sundstrom, C.; Nilsson, K.; Hellman, L.

In: Scandinavian Journal of Immunology, Vol. 39, No. 5, 1994, p. 489-498.

Research output: Contribution to journalArticle

Nilsson, G, Blom, T, Kusche-Gullberg, M, Kjellen, L, Butterfield, JH, Sundstrom, C, Nilsson, K & Hellman, L 1994, 'Phenotypic characterization of the human mast-cell line HMC-1', Scandinavian Journal of Immunology, vol. 39, no. 5, pp. 489-498. https://doi.org/10.1111/j.1365-3083.1994.tb03404.x
Nilsson G, Blom T, Kusche-Gullberg M, Kjellen L, Butterfield JH, Sundstrom C et al. Phenotypic characterization of the human mast-cell line HMC-1. Scandinavian Journal of Immunology. 1994;39(5):489-498. https://doi.org/10.1111/j.1365-3083.1994.tb03404.x
Nilsson, G. ; Blom, T. ; Kusche-Gullberg, M. ; Kjellen, L. ; Butterfield, J. H. ; Sundstrom, C. ; Nilsson, K. ; Hellman, L. / Phenotypic characterization of the human mast-cell line HMC-1. In: Scandinavian Journal of Immunology. 1994 ; Vol. 39, No. 5. pp. 489-498.
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