Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients

Mathew Wiest, Katherine Upchurch, Md Mahmudul Hasan, Jacob Cardenas, Bobby Lanier, Mark Millard, Jacob Turner, SangKon Oh, HyeMee Joo

Research output: Contribution to journalArticle

Abstract

Background: IL-10-producing regulatory B cells (Bregs) are widely ascribed immune regulatory functions. However, Breg subsets in human asthma have not been fully investigated. Objective: We studied Breg subsets in adult allergic asthma patients by assessing two major parameters, frequency and IL-10 expression. We then investigated factors that affect these two parameters in patients. Methods: Peripheral blood mononuclear cells (PBMCs) of adult allergic asthma patients (N = 26) and non-asthmatic controls (N = 28) were used to assess the frequency of five subsets of transitional B cells (TBs), three subsets of CD24highCD27+B cells and B1 cells. In addition to clinical data, IL-10 expression by individual Breg subsets was assessed by flow cytometry. Results: Asthma patients had decreases of CD5+ and CD1d+CD5+, but an increase of CD27+ TBs which was significant in patients with moderate asthma (60 < FEV1 < 80). Regardless of asthma severity, there was no significant alteration in the frequencies of 6 other Breg subsets tested. However, we found that oral corticosteroid (OCS) significantly affected the frequency of Bregs in Breg subset-specific manners. OCS decreased CD5+ and CD1d+CD5+ TBs, but increased CD27+ TBs and CD10+CD24highCD27+ cells. Furthermore, OCS decreased IL-10 expression by CD27+ TBs, all 3 CD24highCD27+ B cell subsets (CD5+, CD10+ and CD1d+) and B1 cells. OCS-mediated inhibition of IL-10 expression was not observed in the other Breg subsets tested. Conclusion & Clinical Relevance: Alterations in the frequency of Bregs and their ability to express IL-10 are Breg subset-specific. OCS treatment significantly affects the frequency as well as their ability to express IL-10 in Breg subset-specific manners.

Original languageEnglish (US)
JournalClinical and Experimental Allergy
DOIs
StatePublished - Jan 1 2019

Fingerprint

Regulatory B-Lymphocytes
B-Lymphocyte Subsets
Interleukin-10
Asthma
Adrenal Cortex Hormones
B-Lymphoid Precursor Cells
Blood Cells
Flow Cytometry

Keywords

  • allergy
  • asthma
  • B1
  • Bregs
  • CD24CD27
  • IL-10
  • oral corticosteroid
  • regulatory B cell
  • transitional

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients. / Wiest, Mathew; Upchurch, Katherine; Hasan, Md Mahmudul; Cardenas, Jacob; Lanier, Bobby; Millard, Mark; Turner, Jacob; Oh, SangKon; Joo, HyeMee.

In: Clinical and Experimental Allergy, 01.01.2019.

Research output: Contribution to journalArticle

Wiest, Mathew ; Upchurch, Katherine ; Hasan, Md Mahmudul ; Cardenas, Jacob ; Lanier, Bobby ; Millard, Mark ; Turner, Jacob ; Oh, SangKon ; Joo, HyeMee. / Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients. In: Clinical and Experimental Allergy. 2019.
@article{ae27dafbab6241c3ae3e8d8296a1d958,
title = "Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients",
abstract = "Background: IL-10-producing regulatory B cells (Bregs) are widely ascribed immune regulatory functions. However, Breg subsets in human asthma have not been fully investigated. Objective: We studied Breg subsets in adult allergic asthma patients by assessing two major parameters, frequency and IL-10 expression. We then investigated factors that affect these two parameters in patients. Methods: Peripheral blood mononuclear cells (PBMCs) of adult allergic asthma patients (N = 26) and non-asthmatic controls (N = 28) were used to assess the frequency of five subsets of transitional B cells (TBs), three subsets of CD24highCD27+B cells and B1 cells. In addition to clinical data, IL-10 expression by individual Breg subsets was assessed by flow cytometry. Results: Asthma patients had decreases of CD5+ and CD1d+CD5+, but an increase of CD27+ TBs which was significant in patients with moderate asthma (60 < FEV1 < 80). Regardless of asthma severity, there was no significant alteration in the frequencies of 6 other Breg subsets tested. However, we found that oral corticosteroid (OCS) significantly affected the frequency of Bregs in Breg subset-specific manners. OCS decreased CD5+ and CD1d+CD5+ TBs, but increased CD27+ TBs and CD10+CD24highCD27+ cells. Furthermore, OCS decreased IL-10 expression by CD27+ TBs, all 3 CD24highCD27+ B cell subsets (CD5+, CD10+ and CD1d+) and B1 cells. OCS-mediated inhibition of IL-10 expression was not observed in the other Breg subsets tested. Conclusion & Clinical Relevance: Alterations in the frequency of Bregs and their ability to express IL-10 are Breg subset-specific. OCS treatment significantly affects the frequency as well as their ability to express IL-10 in Breg subset-specific manners.",
keywords = "allergy, asthma, B1, Bregs, CD24CD27, IL-10, oral corticosteroid, regulatory B cell, transitional",
author = "Mathew Wiest and Katherine Upchurch and Hasan, {Md Mahmudul} and Jacob Cardenas and Bobby Lanier and Mark Millard and Jacob Turner and SangKon Oh and HyeMee Joo",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/cea.13439",
language = "English (US)",
journal = "Clinical and Experimental Allergy",
issn = "0954-7894",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients

AU - Wiest, Mathew

AU - Upchurch, Katherine

AU - Hasan, Md Mahmudul

AU - Cardenas, Jacob

AU - Lanier, Bobby

AU - Millard, Mark

AU - Turner, Jacob

AU - Oh, SangKon

AU - Joo, HyeMee

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: IL-10-producing regulatory B cells (Bregs) are widely ascribed immune regulatory functions. However, Breg subsets in human asthma have not been fully investigated. Objective: We studied Breg subsets in adult allergic asthma patients by assessing two major parameters, frequency and IL-10 expression. We then investigated factors that affect these two parameters in patients. Methods: Peripheral blood mononuclear cells (PBMCs) of adult allergic asthma patients (N = 26) and non-asthmatic controls (N = 28) were used to assess the frequency of five subsets of transitional B cells (TBs), three subsets of CD24highCD27+B cells and B1 cells. In addition to clinical data, IL-10 expression by individual Breg subsets was assessed by flow cytometry. Results: Asthma patients had decreases of CD5+ and CD1d+CD5+, but an increase of CD27+ TBs which was significant in patients with moderate asthma (60 < FEV1 < 80). Regardless of asthma severity, there was no significant alteration in the frequencies of 6 other Breg subsets tested. However, we found that oral corticosteroid (OCS) significantly affected the frequency of Bregs in Breg subset-specific manners. OCS decreased CD5+ and CD1d+CD5+ TBs, but increased CD27+ TBs and CD10+CD24highCD27+ cells. Furthermore, OCS decreased IL-10 expression by CD27+ TBs, all 3 CD24highCD27+ B cell subsets (CD5+, CD10+ and CD1d+) and B1 cells. OCS-mediated inhibition of IL-10 expression was not observed in the other Breg subsets tested. Conclusion & Clinical Relevance: Alterations in the frequency of Bregs and their ability to express IL-10 are Breg subset-specific. OCS treatment significantly affects the frequency as well as their ability to express IL-10 in Breg subset-specific manners.

AB - Background: IL-10-producing regulatory B cells (Bregs) are widely ascribed immune regulatory functions. However, Breg subsets in human asthma have not been fully investigated. Objective: We studied Breg subsets in adult allergic asthma patients by assessing two major parameters, frequency and IL-10 expression. We then investigated factors that affect these two parameters in patients. Methods: Peripheral blood mononuclear cells (PBMCs) of adult allergic asthma patients (N = 26) and non-asthmatic controls (N = 28) were used to assess the frequency of five subsets of transitional B cells (TBs), three subsets of CD24highCD27+B cells and B1 cells. In addition to clinical data, IL-10 expression by individual Breg subsets was assessed by flow cytometry. Results: Asthma patients had decreases of CD5+ and CD1d+CD5+, but an increase of CD27+ TBs which was significant in patients with moderate asthma (60 < FEV1 < 80). Regardless of asthma severity, there was no significant alteration in the frequencies of 6 other Breg subsets tested. However, we found that oral corticosteroid (OCS) significantly affected the frequency of Bregs in Breg subset-specific manners. OCS decreased CD5+ and CD1d+CD5+ TBs, but increased CD27+ TBs and CD10+CD24highCD27+ cells. Furthermore, OCS decreased IL-10 expression by CD27+ TBs, all 3 CD24highCD27+ B cell subsets (CD5+, CD10+ and CD1d+) and B1 cells. OCS-mediated inhibition of IL-10 expression was not observed in the other Breg subsets tested. Conclusion & Clinical Relevance: Alterations in the frequency of Bregs and their ability to express IL-10 are Breg subset-specific. OCS treatment significantly affects the frequency as well as their ability to express IL-10 in Breg subset-specific manners.

KW - allergy

KW - asthma

KW - B1

KW - Bregs

KW - CD24CD27

KW - IL-10

KW - oral corticosteroid

KW - regulatory B cell

KW - transitional

UR - http://www.scopus.com/inward/record.url?scp=85068152526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068152526&partnerID=8YFLogxK

U2 - 10.1111/cea.13439

DO - 10.1111/cea.13439

M3 - Article

C2 - 31132180

AN - SCOPUS:85068152526

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

SN - 0954-7894

ER -