Phenethyl isothiocyanate induces cell cycle arrest and reduction of α- and β-tubulin isotypes in human prostate cancer cells

Ping Yin, Tomoya Kawamura, Meilan He, Donkena Krishna Vanaja, Charles Y.F. Young

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

This study was to investigate the effect of phenethyl isothiocyanate (PEITC), a constituent of many edible cruciferous vegetables, on the expression of α- and β-tubulins, which are the main components of microtubules in prostate cancer cells. Flow cytometry, light microscopy and western blot were used to study the cell cycle distribution, morphology changes and the expression of α- and β-tubulins in prostate cancer cells treated with PEITC. The results showed that PEITC-induced G2-M cell phase arrest and inhibited the expression of α- and β-tubulin proteins in a number of human prostatic carcinoma cell lines. Further, it is showed that this inhibitory effect could be reversed by antioxidant N-acetyl cysteine and proteasome inhibitor MG132. Finally, it is concluded that PEITC inhibited the expression of α- and β-tubulins in prostate cancer cells, which is at least related to the oxygen reaction species and protein degradation.

Original languageEnglish (US)
Pages (from-to)57-64
Number of pages8
JournalCell biology international
Volume33
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Cell cycle
  • Phenethyl isothiocyanate
  • Prostate cancer
  • Tubulin

ASJC Scopus subject areas

  • Cell Biology

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