Pharmacological activation changes stiffness of cultured human airway smooth muscle cells

Rolf D. Hubmayr, Stephanie A. Shore, Jeffrey J. Fredberg, Emanuelle Planus, Reynold A. Panettieri, Winfried Moller, Joachim Heyder, Ning Wang

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

Using magnetic twisting cytometry (MTC), we measured the cytoskeletal stiffness of adherent human airway smooth muscle (HASM) cells. We hypothesized that modulation of actin-myosin interactions by application of contractile agonists would induce changes in cytoskeletal stiffness. In cells plated on high-density collagen, bradykinin (10-6 M) and histamine (10-4 M) increased stiffness by 85 ± 15 and 68 ± 16%, respectively. Increases in cell stiffness were also consistently observed after acetylcholine, substance P, and KCl. The bronchodilator agonists isoproterenol, prostaglandin E2, forskolin, dibutryl adenosine 3',5'-cyclic monophosphate, and 8- bromoguanosine 3',5'-cyclic monophosphate each caused a dose-dependent decrease in cell stiffness in unstimulated as well as bradykinin-treated cells. HASM cells plated on high-density collagen were stiffer than cells plated on low-density collagen (126 ± 16 vs. 43 ± 3 dyn/cm2) and developed more pronounced increases in stiffness in response to bradykinin as well as more pronounced decreases in stiffness in response to isoproterenol. These results are consistent with the hypothesis that modulation of actin-myosin interactions by application of contractile agonists causes changes in cytoskeletal stiffness of HASM cells. MTC may be a valuable tool for evaluating the mechanisms of pharmacomechanical coupling in airway smooth muscle cells in culture.

Original languageEnglish (US)
Pages (from-to)C1660-C1668
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number5 40-5
DOIs
StatePublished - Nov 1996

Keywords

  • cell mechanics
  • cytoskeleton
  • magnetic twisting cytometry
  • signal transduction
  • smooth muscle contraction

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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  • Cite this

    Hubmayr, R. D., Shore, S. A., Fredberg, J. J., Planus, E., Panettieri, R. A., Moller, W., Heyder, J., & Wang, N. (1996). Pharmacological activation changes stiffness of cultured human airway smooth muscle cells. American Journal of Physiology - Cell Physiology, 271(5 40-5), C1660-C1668. https://doi.org/10.1152/ajpcell.1996.271.5.c1660