Phalloidin suppresses force in nebulin-rich lamprey cardiac muscle

A. E. Bukatina, J. Korinek, Gary C Sieck, Marek Belohlavek

Research output: Contribution to journalArticle

Abstract

We assessed the effect of phalloidin, known to detach nebulin from actin in skeletal myofibrils, on the isometric force of skinned lamprey cardiac muscle, which has nebulin in amounts comparable to that in skeletal muscle. In contrast to mammalian cardiac muscle, which contains much less nebulin and reacts to phalloidin only by an increase in force, the lamprey cardiac muscle responds to phalloidin by a pronounced (∼ 50%) reduction in isometric force, thereby resembling the behavior of skeletal muscle. These results support our hypothesis that nebulin detachment from actin underlies phalloidin-induced force loss and suggest a role of actin-nebulin interaction in contractile function.

Original languageEnglish (US)
Pages (from-to)789-792
Number of pages4
JournalBiophysics
Volume51
Issue number5
DOIs
StatePublished - Oct 2006

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Lampreys
Phalloidine
Myocardium
Actins
Skeletal Muscle
Myofibrils
nebulin

Keywords

  • Actin
  • Cardiac muscle
  • Contraction regulation
  • Cytoskeleton
  • Lamprey
  • Myofibrillar proteins
  • Nebulin
  • Phalloidin
  • Skeletal muscle

ASJC Scopus subject areas

  • Biophysics

Cite this

Phalloidin suppresses force in nebulin-rich lamprey cardiac muscle. / Bukatina, A. E.; Korinek, J.; Sieck, Gary C; Belohlavek, Marek.

In: Biophysics, Vol. 51, No. 5, 10.2006, p. 789-792.

Research output: Contribution to journalArticle

Bukatina, A. E. ; Korinek, J. ; Sieck, Gary C ; Belohlavek, Marek. / Phalloidin suppresses force in nebulin-rich lamprey cardiac muscle. In: Biophysics. 2006 ; Vol. 51, No. 5. pp. 789-792.
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