Phage display of adenoviral HI loop for "context-specific" ligand selection

D. Ghosh, M. A. Barry

Research output: Contribution to journalConference article

Abstract

Adenoviruses are used as delivery vectors in gene therapy mainly due to their ability to efficiently infect dividing and non-dividing cells. However, their success is hindered due to its tropism for non-targeted cell types. Adenoviruses can be genetically retargeted by ligand addition to confer specificity. Phage-presenting random peptide libraries have been used to select potential ligands. This approach does not require knowledge of the cell biology. However, selected peptides engineered into the adenovirus can reduce viral capsid function, and the adenovirus can impair targeting ability of the selected peptide. Our research focuses on the development of "context-specific" random libraries for ligand selection. By introducing a viral context, we hope to select a peptide that can be introduced into the virus without affecting function. In this paper, we report the phage display of biotin acceptor peptide within the adenoviral HI loop (denoted HI-BAP). HI-BAP is expressed and enzymatically biotinylated on the pIII minor coat protein of filamentous phage. This approach can improve targeting of viral vectors.

Original languageEnglish (US)
Pages (from-to)555-556
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Keywords

  • Gene therapy
  • HI loop
  • Phage display
  • Targeting

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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