PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex

William P. Lafuse, Dennis Lanning, Thomas Spies, Chella S. David

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have constructed a long range restriction map of the S/D segment of the mouse H-2 complex by pulsed field gel electrophoresis and hybridization with mouse cDNA probes to Bf and Tnfa genes and human cDNA probes to BAT2, BAT3, BAT4, BAT5, and BAT6 genes which have recently been mapped to the human HLA complex between C2 and HLA-B. The distance between the mouse C2 and Tnfa genes was found to be approximately 350 kilobases. The position of the mouse Bat genes in this map were found to be comparable to the position of the BAT genes in the human HLA complex. A panel of recombinant mouse strains was also examined by restriction fragment analysis with probes detecting the Hsp70, Bat5, and Tnfa genes. The results indicate that recombination in this segment is not random. No recombinants were found with crossovers between the C2 and Hsp70 genes and only one recombinant was found with a crossover between Tnfa and H-2D. In contrast, the crossover sites of 16 recombinants were mapped between the Hsp70 and Tnfa genes. Seven of these recombinants were found to have crossovers between Hsp70 and Bat5 and three recombinants were found to have crossover sites between Bat5 and Tnfa.

Original languageEnglish (US)
Pages (from-to)110-116
Number of pages7
JournalImmunogenetics
Volume36
Issue number2
DOIs
StatePublished - Jun 1992

Fingerprint

Restriction Fragment Length Polymorphisms
Genes
Complementary DNA
Gene Order
HLA-B Antigens
Pulsed Field Gel Electrophoresis
Genetic Recombination

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Lafuse, W. P., Lanning, D., Spies, T., & David, C. S. (1992). PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex. Immunogenetics, 36(2), 110-116. https://doi.org/10.1007/BF00215287

PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex. / Lafuse, William P.; Lanning, Dennis; Spies, Thomas; David, Chella S.

In: Immunogenetics, Vol. 36, No. 2, 06.1992, p. 110-116.

Research output: Contribution to journalArticle

Lafuse, WP, Lanning, D, Spies, T & David, CS 1992, 'PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex', Immunogenetics, vol. 36, no. 2, pp. 110-116. https://doi.org/10.1007/BF00215287
Lafuse, William P. ; Lanning, Dennis ; Spies, Thomas ; David, Chella S. / PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex. In: Immunogenetics. 1992 ; Vol. 36, No. 2. pp. 110-116.
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