Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1

Mohamad H. Yamani, Norman B. Ratliff, Daniel J. Cook, E. Murat Tuzcu, Yang Yu, Robert Hobbs, Gustavo Rincon, Corinne Bott-Silverman, James B. Young, Nicholas Smedira, Randall C. Starling

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Abstract

OBJECTIVES: We evaluated chimerism and stromal cell-derived factor-1 (SDF-1) expression in response to peritransplant ischemic injury following human heart transplantation. BACKGROUND: Myocardial ischemia has been shown to trigger mobilization of stem cells to the heart in animal experiments. METHODS: Between January 1998 and April 2002, a total of 114 male recipients received hearts from female donors. Of these 114 recipients, 26 had evidence of ischemic injury on their initial heart biopsies (ischemia group). These were compared to the remaining 88 patients (control group). Heart biopsy specimens obtained initially at one week and at one year after transplant were evaluated from 20 matched patients of each group for the presence of Y chromosome-containing nuclei. The SDF-1 messenger ribonucleic acid (mRNA) and protein expression were also evaluated on initial heart biopsy specimens. RESULTS: At one week, Y chromosome-containing nuclei were significantly increased in the ischemia group (0.68% vs. 0.04%; p < 0.0001) compared to the control group. These were positive for the stem cell factor receptor c-kit. A significant 3.3-fold increased mRNA expression (p = 0.001) and 2.8-fold increased protein expression (p = 0.01) of SDF-1 was noted in the ischemia group. At one year, Y chromosome was detected in 0.29% of cardiomyocyte nuclei in the ischemia group but none in the control group. The ischemia group had poorer survival and increased vasculopathy. CONCLUSIONS: This is the first report to describe chimerism and up-regulation of SDF-1 in human heart transplantation in response to ischemic injury.

Original languageEnglish (US)
Pages (from-to)1029-1035
Number of pages7
JournalJournal of the American College of Cardiology
Volume46
Issue number6
DOIs
StatePublished - Sep 20 2005
Externally publishedYes

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Chemokine CXCL12
Up-Regulation
Ischemia
Y Chromosome
Wounds and Injuries
Chimerism
Heart Transplantation
Biopsy
Control Groups
RNA
Hematopoietic Stem Cell Mobilization
Proto-Oncogene Proteins c-kit
Cardiac Myocytes
Myocardial Ischemia
Proteins
Tissue Donors
Transplants
Survival

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Yamani, M. H., Ratliff, N. B., Cook, D. J., Tuzcu, E. M., Yu, Y., Hobbs, R., ... Starling, R. C. (2005). Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1. Journal of the American College of Cardiology, 46(6), 1029-1035. https://doi.org/10.1016/j.jacc.2005.04.059

Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1. / Yamani, Mohamad H.; Ratliff, Norman B.; Cook, Daniel J.; Tuzcu, E. Murat; Yu, Yang; Hobbs, Robert; Rincon, Gustavo; Bott-Silverman, Corinne; Young, James B.; Smedira, Nicholas; Starling, Randall C.

In: Journal of the American College of Cardiology, Vol. 46, No. 6, 20.09.2005, p. 1029-1035.

Research output: Contribution to journalArticle

Yamani, MH, Ratliff, NB, Cook, DJ, Tuzcu, EM, Yu, Y, Hobbs, R, Rincon, G, Bott-Silverman, C, Young, JB, Smedira, N & Starling, RC 2005, 'Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1', Journal of the American College of Cardiology, vol. 46, no. 6, pp. 1029-1035. https://doi.org/10.1016/j.jacc.2005.04.059
Yamani, Mohamad H. ; Ratliff, Norman B. ; Cook, Daniel J. ; Tuzcu, E. Murat ; Yu, Yang ; Hobbs, Robert ; Rincon, Gustavo ; Bott-Silverman, Corinne ; Young, James B. ; Smedira, Nicholas ; Starling, Randall C. / Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1. In: Journal of the American College of Cardiology. 2005 ; Vol. 46, No. 6. pp. 1029-1035.
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abstract = "OBJECTIVES: We evaluated chimerism and stromal cell-derived factor-1 (SDF-1) expression in response to peritransplant ischemic injury following human heart transplantation. BACKGROUND: Myocardial ischemia has been shown to trigger mobilization of stem cells to the heart in animal experiments. METHODS: Between January 1998 and April 2002, a total of 114 male recipients received hearts from female donors. Of these 114 recipients, 26 had evidence of ischemic injury on their initial heart biopsies (ischemia group). These were compared to the remaining 88 patients (control group). Heart biopsy specimens obtained initially at one week and at one year after transplant were evaluated from 20 matched patients of each group for the presence of Y chromosome-containing nuclei. The SDF-1 messenger ribonucleic acid (mRNA) and protein expression were also evaluated on initial heart biopsy specimens. RESULTS: At one week, Y chromosome-containing nuclei were significantly increased in the ischemia group (0.68{\%} vs. 0.04{\%}; p < 0.0001) compared to the control group. These were positive for the stem cell factor receptor c-kit. A significant 3.3-fold increased mRNA expression (p = 0.001) and 2.8-fold increased protein expression (p = 0.01) of SDF-1 was noted in the ischemia group. At one year, Y chromosome was detected in 0.29{\%} of cardiomyocyte nuclei in the ischemia group but none in the control group. The ischemia group had poorer survival and increased vasculopathy. CONCLUSIONS: This is the first report to describe chimerism and up-regulation of SDF-1 in human heart transplantation in response to ischemic injury.",
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T1 - Peritransplant ischemic injury is associated with up-regulation of stromal cell-derived factor-1

AU - Yamani, Mohamad H.

AU - Ratliff, Norman B.

AU - Cook, Daniel J.

AU - Tuzcu, E. Murat

AU - Yu, Yang

AU - Hobbs, Robert

AU - Rincon, Gustavo

AU - Bott-Silverman, Corinne

AU - Young, James B.

AU - Smedira, Nicholas

AU - Starling, Randall C.

PY - 2005/9/20

Y1 - 2005/9/20

N2 - OBJECTIVES: We evaluated chimerism and stromal cell-derived factor-1 (SDF-1) expression in response to peritransplant ischemic injury following human heart transplantation. BACKGROUND: Myocardial ischemia has been shown to trigger mobilization of stem cells to the heart in animal experiments. METHODS: Between January 1998 and April 2002, a total of 114 male recipients received hearts from female donors. Of these 114 recipients, 26 had evidence of ischemic injury on their initial heart biopsies (ischemia group). These were compared to the remaining 88 patients (control group). Heart biopsy specimens obtained initially at one week and at one year after transplant were evaluated from 20 matched patients of each group for the presence of Y chromosome-containing nuclei. The SDF-1 messenger ribonucleic acid (mRNA) and protein expression were also evaluated on initial heart biopsy specimens. RESULTS: At one week, Y chromosome-containing nuclei were significantly increased in the ischemia group (0.68% vs. 0.04%; p < 0.0001) compared to the control group. These were positive for the stem cell factor receptor c-kit. A significant 3.3-fold increased mRNA expression (p = 0.001) and 2.8-fold increased protein expression (p = 0.01) of SDF-1 was noted in the ischemia group. At one year, Y chromosome was detected in 0.29% of cardiomyocyte nuclei in the ischemia group but none in the control group. The ischemia group had poorer survival and increased vasculopathy. CONCLUSIONS: This is the first report to describe chimerism and up-regulation of SDF-1 in human heart transplantation in response to ischemic injury.

AB - OBJECTIVES: We evaluated chimerism and stromal cell-derived factor-1 (SDF-1) expression in response to peritransplant ischemic injury following human heart transplantation. BACKGROUND: Myocardial ischemia has been shown to trigger mobilization of stem cells to the heart in animal experiments. METHODS: Between January 1998 and April 2002, a total of 114 male recipients received hearts from female donors. Of these 114 recipients, 26 had evidence of ischemic injury on their initial heart biopsies (ischemia group). These were compared to the remaining 88 patients (control group). Heart biopsy specimens obtained initially at one week and at one year after transplant were evaluated from 20 matched patients of each group for the presence of Y chromosome-containing nuclei. The SDF-1 messenger ribonucleic acid (mRNA) and protein expression were also evaluated on initial heart biopsy specimens. RESULTS: At one week, Y chromosome-containing nuclei were significantly increased in the ischemia group (0.68% vs. 0.04%; p < 0.0001) compared to the control group. These were positive for the stem cell factor receptor c-kit. A significant 3.3-fold increased mRNA expression (p = 0.001) and 2.8-fold increased protein expression (p = 0.01) of SDF-1 was noted in the ischemia group. At one year, Y chromosome was detected in 0.29% of cardiomyocyte nuclei in the ischemia group but none in the control group. The ischemia group had poorer survival and increased vasculopathy. CONCLUSIONS: This is the first report to describe chimerism and up-regulation of SDF-1 in human heart transplantation in response to ischemic injury.

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