Peripheral nerve microenvironment: Collection of endoneurially enriched fluid

Phillip A. Low; Jeffrey K. Yao; Joseph F. Poduslo; David E. Donald; Peter J. Dyck

doi:10.1016/0014-4886(82)90154-6

Peripheral nerve microenvironment: Collection of endoneurially enriched fluid

Phillip A. Low, Jeffrey K. Yao, Joseph F. Poduslo, David E. Donald, Peter J. Dyck

Neurology

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Abstract

We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 10⁴ to 2 × 10⁵ of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.

Original language	English (US)
Pages (from-to)	208-214
Number of pages	7
Journal	Experimental Neurology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/0014-4886(82)90154-6
State	Published - Jul 1982

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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@article{d0e51b79546e491286aacac731209ef5,

title = "Peripheral nerve microenvironment: Collection of endoneurially enriched fluid",

abstract = "We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 104 to 2 × 105 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.",

author = "Low, {Phillip A.} and Yao, {Jeffrey K.} and Poduslo, {Joseph F.} and Donald, {David E.} and Dyck, {Peter J.}",

note = "Funding Information: Abbreviations: EEF-endoneurially enriched fluid, EFP-endoneurial fluid pressure, EFOP-endoneurial fluid osmotic pressure, SDS-PGE-sodium dodecyl sulfate-pore gradient electrophoresis. {\textquoteright} This work was supported in part by Center grants from the National Institutes of Health (NS 14304) and the Muscular Dystrophy Association and Mayo, Borchard, Upton and Gal-lagher Funds. Please send correspondence to Dr. P. A. Low, Peripheral Nerve Laboratory, Department of Neurology, Mayo Clinic, Rochester, MN 55901.",

year = "1982",

month = jul,

doi = "10.1016/0014-4886(82)90154-6",

language = "English (US)",

volume = "77",

pages = "208--214",

journal = "Experimental Neurology",

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publisher = "Academic Press Inc.",

number = "1",

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T1 - Peripheral nerve microenvironment

T2 - Collection of endoneurially enriched fluid

AU - Low, Phillip A.

AU - Yao, Jeffrey K.

AU - Poduslo, Joseph F.

AU - Donald, David E.

AU - Dyck, Peter J.

N1 - Funding Information: Abbreviations: EEF-endoneurially enriched fluid, EFP-endoneurial fluid pressure, EFOP-endoneurial fluid osmotic pressure, SDS-PGE-sodium dodecyl sulfate-pore gradient electrophoresis. ’ This work was supported in part by Center grants from the National Institutes of Health (NS 14304) and the Muscular Dystrophy Association and Mayo, Borchard, Upton and Gal-lagher Funds. Please send correspondence to Dr. P. A. Low, Peripheral Nerve Laboratory, Department of Neurology, Mayo Clinic, Rochester, MN 55901.

PY - 1982/7

Y1 - 1982/7

N2 - We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 104 to 2 × 105 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.

AB - We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 104 to 2 × 105 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.

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JO - Experimental Neurology

JF - Experimental Neurology

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Peripheral nerve microenvironment: Collection of endoneurially enriched fluid

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