Peripheral nerve microenvironment: Collection of endoneurially enriched fluid

Phillip Anson Low, Jeffrey K. Yao, Joseph F. Poduslo, David E. Donald, Peter J Dyck

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 104 to 2 × 105 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.

Original languageEnglish (US)
Pages (from-to)208-214
Number of pages7
JournalExperimental Neurology
Volume77
Issue number1
DOIs
StatePublished - 1982

Fingerprint

Peripheral Nerves
Proteins
Protein Array Analysis
Polyethylene
Sciatic Nerve
Serum
Sodium Dodecyl Sulfate
Capsules
Electrophoresis
Albumins
Molecular Weight
Lead

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Peripheral nerve microenvironment : Collection of endoneurially enriched fluid. / Low, Phillip Anson; Yao, Jeffrey K.; Poduslo, Joseph F.; Donald, David E.; Dyck, Peter J.

In: Experimental Neurology, Vol. 77, No. 1, 1982, p. 208-214.

Research output: Contribution to journalArticle

Low, Phillip Anson ; Yao, Jeffrey K. ; Poduslo, Joseph F. ; Donald, David E. ; Dyck, Peter J. / Peripheral nerve microenvironment : Collection of endoneurially enriched fluid. In: Experimental Neurology. 1982 ; Vol. 77, No. 1. pp. 208-214.
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