Peripheral axotomy induces neurofilament decrease, atrophy, demyelination and degeneration of root and fasciculus gracilis fibers

Peter J Dyck, Alfred Lais, Jeannine Karnes, Margaret Sparks, P. James B Dyck

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have recently shown that peripheral axotomy by hindlimb amputation in adult cats sequentially results in neurofilament and microtubule decrease and axonal atrophy, myelin wrinkling, myelin remodeling (de- and remyelination), more atrophy and axonal degeneration in proximal sciatic and L7 segmental nerve fibers. The neurophatologic, morphometric and teased fiber alterations in the myelinated fibers (MF) of roots and sampled levels of fasciculus gracilis in groups of adult cats 24 months after hindlimb amputation have now been studied. We found: (1) a severe decrease of neurofilaments, axonal atrophy, myelin wrinkling, de- and remyelination and axonal loss in posterior root axons; (2) that these morphologic abnormalities extended up the fasciculus gracilis in the appropriate territories established from degenerative studies; (3) that the retrograde effect was less severe in ventral root fibers, although atrophy and sprouting were demonstrated here, and (4) that the cellular sequence of retrograde atrophic degeneration of ascending axons was similar to that observed in proximal stump axons. These findings confirm that primary afferent neurons are more vulnerable to axotomy than lower motot neurons and may provide an additional explanation for the poorer functional restoration of sensory than of motor deficit after root compression and in delayed nerve reconnection. Our observations also have important implications for interpretation of neuropathologic alterations in roots and fasciculus gracilis, since the observed features may be secondary to axotomy of peripheral nerve fibers induced by disease and not evidence of a primary derangement.

Original languageEnglish (US)
Pages (from-to)19-36
Number of pages18
JournalBrain Research
Volume340
Issue number1
DOIs
StatePublished - Aug 5 1985

Fingerprint

Axotomy
Intermediate Filaments
Demyelinating Diseases
Atrophy
Myelin Sheath
Axons
Hindlimb
Amputation
Nerve Fibers
Cats
Retrograde Degeneration
Afferent Neurons
Spinal Nerve Roots
Peripheral Nerves
Microtubules
Neurons

Keywords

  • atrophic degeneration
  • axotomy
  • microtubule decrease
  • neurofilament decrease
  • neuropathologic alteration of roots and fasciculus gracilis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Peripheral axotomy induces neurofilament decrease, atrophy, demyelination and degeneration of root and fasciculus gracilis fibers. / Dyck, Peter J; Lais, Alfred; Karnes, Jeannine; Sparks, Margaret; Dyck, P. James B.

In: Brain Research, Vol. 340, No. 1, 05.08.1985, p. 19-36.

Research output: Contribution to journalArticle

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