TY - JOUR
T1 - Performance of flow cytometry to screen urine for bacteria and white blood cells prior to urine culture
AU - Giesen, Callen D.
AU - Greeno, Amanda M.
AU - Thompson, Katherine A.
AU - Patel, Robin
AU - Jenkins, Sarah M.
AU - Lieske, John C.
N1 - Funding Information:
The authors thank Dr. Timothy Larson (Mayo Clinic Renal Laboratory) and Paul Isensee (Sysmex, Inc.) for helpful discussions, and the numerous Mayo Clinic Renal Laboratory personnel who helped aliquot specimens and analyze Gram stains. This study was funded solely by the Mayo Foundation .
PY - 2013/6
Y1 - 2013/6
N2 - Background: The gold standard test for the diagnosis of urinary tract infection is bacterial culture. However, urine cultures are labor intensive and costly. Furthermore, since results take 1-2. days many patients are treated presumptively prior to culture results being known. Methods: We evaluated the Sysmex UF-1000i for the quantification of bacteria and white blood cells (WBCs) in urine in order to determine if it could be used to predict positive culture in comparison to the use of gram stain as a screening tool. Results: The UF-1000i demonstrated good linearity, within and between run precision for bacterial and WBC quantification. Using ROC analysis, the AUC for predicting a positive culture (>105cfu/mL) was 0.95 and 0.90 for bacteria and WBCs, respectively, with optimum cutoffs of 288.9bacteria/μL and 31.8WBCs/μL, respectively. At these cutoffs, sensitivity (SE) and specificity (SP) for culture positivity were 0.93 and 0.86, respectively, for bacterial counts and 0.89 and 0.79, respectively, for WBC counts. The use of gender specific bacterial cutoffs improved performance, especially in males. In comparison, SE and SP of urine Gram stain were 0.94 and 0.68, respectively. Conclusions: Quantification of bacteria in unspun urine samples by the Sysmex UF-1000i can be used to screen urine samples for those likely to grow >105cfu/mL. The Sysmex UF-1000i demonstrated increased SP over urine Gram stain, and in this study population could reduce unnecessary reflex urine cultures by 55%.
AB - Background: The gold standard test for the diagnosis of urinary tract infection is bacterial culture. However, urine cultures are labor intensive and costly. Furthermore, since results take 1-2. days many patients are treated presumptively prior to culture results being known. Methods: We evaluated the Sysmex UF-1000i for the quantification of bacteria and white blood cells (WBCs) in urine in order to determine if it could be used to predict positive culture in comparison to the use of gram stain as a screening tool. Results: The UF-1000i demonstrated good linearity, within and between run precision for bacterial and WBC quantification. Using ROC analysis, the AUC for predicting a positive culture (>105cfu/mL) was 0.95 and 0.90 for bacteria and WBCs, respectively, with optimum cutoffs of 288.9bacteria/μL and 31.8WBCs/μL, respectively. At these cutoffs, sensitivity (SE) and specificity (SP) for culture positivity were 0.93 and 0.86, respectively, for bacterial counts and 0.89 and 0.79, respectively, for WBC counts. The use of gender specific bacterial cutoffs improved performance, especially in males. In comparison, SE and SP of urine Gram stain were 0.94 and 0.68, respectively. Conclusions: Quantification of bacteria in unspun urine samples by the Sysmex UF-1000i can be used to screen urine samples for those likely to grow >105cfu/mL. The Sysmex UF-1000i demonstrated increased SP over urine Gram stain, and in this study population could reduce unnecessary reflex urine cultures by 55%.
KW - Flow cytometry
KW - Gram stain
KW - Sysmex UF-1000i
KW - Urinary tract infection
KW - Urine culture
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U2 - 10.1016/j.clinbiochem.2013.03.005
DO - 10.1016/j.clinbiochem.2013.03.005
M3 - Article
C2 - 23500010
AN - SCOPUS:84877925480
SN - 0009-9120
VL - 46
SP - 810
EP - 813
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 9
ER -