Peptide Nucleic Acids Targeted to the Amyloid Precursor Protein

Beth M. McMahon, Jennifer Stewart, Abdul Fauq, Steven G Younkin, Linda Younkin, Elliott Richelson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The depositing in brain of amyloid β peptide (Aβ), which is formed by the cleavage of amyloid precursor protein (APP), is likely an etiologic factor in Alzheimer's disease (AD). Of the different forms of Aβ, Aβ(1-42) causes fibril formation and increases aggregation at elevated levels, which can lead to neuronal death. It is hypothesized that if the levels of Aβ, particularly Aβ(1-42), were reduced, then the onset of AD would be slowed or possibly prevented. Therefore, we are using peptide nucleic acids (PNAs) targeted to APP, as well as other key proteins, to try to decrease plasma and brain levels of Aβ(1-40) and Aβ(1-42). This research project was designed to utilize the expertise of our laboratory in the use of PNAs, a third-generation antisense or antigene molecule, to knock down proteins in brain. Antisense compounds specifically knock down the expression of a particular protein by inhibiting translation at the level of mRNA. On the other hand, antigene compounds knock down expression at the level of transcription. For experiments involving antisense strategies, there are several advantages to using PNAs as opposed to the traditional oligonucleotide molecules. We report here the ongoing studies with mice and rats with PNAs targeting APP, as well as BACE.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalJournal of Molecular Neuroscience
Volume20
Issue number3
DOIs
StatePublished - 2003

Fingerprint

Peptide Nucleic Acids
Amyloid beta-Protein Precursor
Brain
Alzheimer Disease
Proteins
Molecules
Protein Biosynthesis
Transcription
Amyloid
Oligonucleotides
Rats
Agglomeration
Plasmas
Messenger RNA
Research
Experiments

Keywords

  • Antigene
  • Antisense
  • Mouse
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Neuroscience(all)

Cite this

Peptide Nucleic Acids Targeted to the Amyloid Precursor Protein. / McMahon, Beth M.; Stewart, Jennifer; Fauq, Abdul; Younkin, Steven G; Younkin, Linda; Richelson, Elliott.

In: Journal of Molecular Neuroscience, Vol. 20, No. 3, 2003, p. 261-265.

Research output: Contribution to journalArticle

McMahon, BM, Stewart, J, Fauq, A, Younkin, SG, Younkin, L & Richelson, E 2003, 'Peptide Nucleic Acids Targeted to the Amyloid Precursor Protein', Journal of Molecular Neuroscience, vol. 20, no. 3, pp. 261-265. https://doi.org/10.1385/JMN:20:3:261
McMahon, Beth M. ; Stewart, Jennifer ; Fauq, Abdul ; Younkin, Steven G ; Younkin, Linda ; Richelson, Elliott. / Peptide Nucleic Acids Targeted to the Amyloid Precursor Protein. In: Journal of Molecular Neuroscience. 2003 ; Vol. 20, No. 3. pp. 261-265.
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