Peptide binding α1α2 domain of HLA-B27 contributes to the disease pathogenesis in transgenie mice

Sanjay D. Khare, Sonwoo Lee, Michael J. Bull, Julie Hanson, Harvinder S. Luthra, Gunther J. Hammerling, Chella S. David

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Human spondyloarthropathies are strongly associated with a major histocompatibility complex (MHC) class I allele, HLA-B27. HLA-B27 transgenic mice and rats demonstrate many features of these diseases further confirming the role of HLA-B27 in disease. Yet the exact role of this molecule in disease pathogenesis is not clearly understood. We have previously reported spontaneous arthritis and nail disease in HLA-B27 transgenic mice lacking β2-microglobulin (B27+β2m°). These observations along with binding studies of B27 derived peptides to HLA-B27 molecule itself led to two hypotheses: (i) HLA-B27 derived peptide as a source of autoantigen; and (ii) HLA-B27 functions as an antigen presenting molecule. In this report. We confirm spontaneous disease in transgenic mice expressing a hybrid B27 molecule with α1α2 domain of B27 and α3 domain of mouse H-2K(d). These mice developed spontaneous arthritis and nail disease when transferred from specific pathogen free barrier facility to the conventional area. Other control mice with MHC class I transgene (e.g., HLA-B7, HLA-Cw3, and H2-D(d)) did not develop such disease. In a MHC reassembly assay, binding of similar peptides to both wild type and hybrid B27 molecules was observed. In addition, the hybrid B27 molecule lacks at least one of the 3 proposed peptides from the third hypervariable (HV3) region of HLA-B27. These data strongly suggest that HLA-B27 molecule is an antigen presenting molecule rather than a peptide donor in the disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)116-126
Number of pages11
JournalHuman Immunology
Volume60
Issue number2
DOIs
StatePublished - Feb 1 1999

Keywords

  • HLA-B27
  • Reactive arthritis
  • Reiter's disease
  • Spondyloarthropathies
  • Transgenic animal model

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Khare, S. D., Lee, S., Bull, M. J., Hanson, J., Luthra, H. S., Hammerling, G. J., & David, C. S. (1999). Peptide binding α1α2 domain of HLA-B27 contributes to the disease pathogenesis in transgenie mice. Human Immunology, 60(2), 116-126. https://doi.org/10.1016/S0198-8859(98)00104-9