Peptide Absorption After Massive Proximal Small Bowel Resection

Mechanisms of Ileal Adaptation

Hisham G. Qandeel, Fernando Alonso, David J. Hernandez, Srivats Madhavan, Judith A. Duenes, Ye Zheng, Michael G. Sarr

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Protein absorption occurs as di- and tri-peptides via H +/peptide co-transporter-1 (PepT1). Aim: The aim of this study is to identify mechanisms of ileal adaptation after massive proximal enterectomy. Hypothesis: Ileal adaptation in uptake of peptides is mediated through upregulation of PepT1 gene expression. Study Design: Rats underwent 70% jejunoileal resection. Total mucosal cellular levels of messenger RNA (mRNA) and protein and transporter-mediated uptake per centimeter of the di-peptide glycyl-sarcosine (Gly-Sar) were compared in remnant ileum 1 and 4 weeks postoperatively to control and to 1-week sham laparotomy rats. Histomorphology, food consumption, and weights of rats were monitored. Results: After 70% resection, although mRNA per cell for PepT1 decreased at 1 week (p = 0. 002), expression of mRNA at 4 weeks and protein at 1 and 4 weeks in remnant ileum were unchanged (p > 0. 1). Ileal Gly-Sar uptake (V max-nanomoles per centimeter per minute, i. e., number of transporters per centimeter) increased at 1 and 4 weeks compared to control and 1-week sham (p < 0. 05 each); K m (i. e., transporter function) was unchanged. Villous heights (millimeters) in remnant ileum increased at 1- and 4-week time points over controls (0. 45 and 0. 57 vs 0. 21, resp; p < 0. 001). Conclusions: Ileal adaptation to proximal resection for peptide absorption occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of PepT1 mRNA or protein per enterocyte.

Original languageEnglish (US)
Pages (from-to)1537-1547
Number of pages11
JournalJournal of Gastrointestinal Surgery
Volume15
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

Symporters
Peptides
Ileum
Sarcosine
Messenger RNA
Proteins
Up-Regulation
Enterocytes
Laparotomy
Hyperplasia
Cell Proliferation
Gene Expression
Weights and Measures
Food
peptide permease

Keywords

  • Adaptation
  • PepT1
  • Peptide absorption
  • Short bowel syndrome
  • Small bowel resection

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Qandeel, H. G., Alonso, F., Hernandez, D. J., Madhavan, S., Duenes, J. A., Zheng, Y., & Sarr, M. G. (2011). Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation. Journal of Gastrointestinal Surgery, 15(9), 1537-1547. https://doi.org/10.1007/s11605-011-1581-z

Peptide Absorption After Massive Proximal Small Bowel Resection : Mechanisms of Ileal Adaptation. / Qandeel, Hisham G.; Alonso, Fernando; Hernandez, David J.; Madhavan, Srivats; Duenes, Judith A.; Zheng, Ye; Sarr, Michael G.

In: Journal of Gastrointestinal Surgery, Vol. 15, No. 9, 09.2011, p. 1537-1547.

Research output: Contribution to journalArticle

Qandeel, HG, Alonso, F, Hernandez, DJ, Madhavan, S, Duenes, JA, Zheng, Y & Sarr, MG 2011, 'Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation', Journal of Gastrointestinal Surgery, vol. 15, no. 9, pp. 1537-1547. https://doi.org/10.1007/s11605-011-1581-z
Qandeel, Hisham G. ; Alonso, Fernando ; Hernandez, David J. ; Madhavan, Srivats ; Duenes, Judith A. ; Zheng, Ye ; Sarr, Michael G. / Peptide Absorption After Massive Proximal Small Bowel Resection : Mechanisms of Ileal Adaptation. In: Journal of Gastrointestinal Surgery. 2011 ; Vol. 15, No. 9. pp. 1537-1547.
@article{45d9662aa5c0407aafaa8b6cdaca4dc9,
title = "Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation",
abstract = "Background: Protein absorption occurs as di- and tri-peptides via H +/peptide co-transporter-1 (PepT1). Aim: The aim of this study is to identify mechanisms of ileal adaptation after massive proximal enterectomy. Hypothesis: Ileal adaptation in uptake of peptides is mediated through upregulation of PepT1 gene expression. Study Design: Rats underwent 70{\%} jejunoileal resection. Total mucosal cellular levels of messenger RNA (mRNA) and protein and transporter-mediated uptake per centimeter of the di-peptide glycyl-sarcosine (Gly-Sar) were compared in remnant ileum 1 and 4 weeks postoperatively to control and to 1-week sham laparotomy rats. Histomorphology, food consumption, and weights of rats were monitored. Results: After 70{\%} resection, although mRNA per cell for PepT1 decreased at 1 week (p = 0. 002), expression of mRNA at 4 weeks and protein at 1 and 4 weeks in remnant ileum were unchanged (p > 0. 1). Ileal Gly-Sar uptake (V max-nanomoles per centimeter per minute, i. e., number of transporters per centimeter) increased at 1 and 4 weeks compared to control and 1-week sham (p < 0. 05 each); K m (i. e., transporter function) was unchanged. Villous heights (millimeters) in remnant ileum increased at 1- and 4-week time points over controls (0. 45 and 0. 57 vs 0. 21, resp; p < 0. 001). Conclusions: Ileal adaptation to proximal resection for peptide absorption occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of PepT1 mRNA or protein per enterocyte.",
keywords = "Adaptation, PepT1, Peptide absorption, Short bowel syndrome, Small bowel resection",
author = "Qandeel, {Hisham G.} and Fernando Alonso and Hernandez, {David J.} and Srivats Madhavan and Duenes, {Judith A.} and Ye Zheng and Sarr, {Michael G.}",
year = "2011",
month = "9",
doi = "10.1007/s11605-011-1581-z",
language = "English (US)",
volume = "15",
pages = "1537--1547",
journal = "Journal of Gastrointestinal Surgery",
issn = "1091-255X",
publisher = "Springer New York",
number = "9",

}

TY - JOUR

T1 - Peptide Absorption After Massive Proximal Small Bowel Resection

T2 - Mechanisms of Ileal Adaptation

AU - Qandeel, Hisham G.

AU - Alonso, Fernando

AU - Hernandez, David J.

AU - Madhavan, Srivats

AU - Duenes, Judith A.

AU - Zheng, Ye

AU - Sarr, Michael G.

PY - 2011/9

Y1 - 2011/9

N2 - Background: Protein absorption occurs as di- and tri-peptides via H +/peptide co-transporter-1 (PepT1). Aim: The aim of this study is to identify mechanisms of ileal adaptation after massive proximal enterectomy. Hypothesis: Ileal adaptation in uptake of peptides is mediated through upregulation of PepT1 gene expression. Study Design: Rats underwent 70% jejunoileal resection. Total mucosal cellular levels of messenger RNA (mRNA) and protein and transporter-mediated uptake per centimeter of the di-peptide glycyl-sarcosine (Gly-Sar) were compared in remnant ileum 1 and 4 weeks postoperatively to control and to 1-week sham laparotomy rats. Histomorphology, food consumption, and weights of rats were monitored. Results: After 70% resection, although mRNA per cell for PepT1 decreased at 1 week (p = 0. 002), expression of mRNA at 4 weeks and protein at 1 and 4 weeks in remnant ileum were unchanged (p > 0. 1). Ileal Gly-Sar uptake (V max-nanomoles per centimeter per minute, i. e., number of transporters per centimeter) increased at 1 and 4 weeks compared to control and 1-week sham (p < 0. 05 each); K m (i. e., transporter function) was unchanged. Villous heights (millimeters) in remnant ileum increased at 1- and 4-week time points over controls (0. 45 and 0. 57 vs 0. 21, resp; p < 0. 001). Conclusions: Ileal adaptation to proximal resection for peptide absorption occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of PepT1 mRNA or protein per enterocyte.

AB - Background: Protein absorption occurs as di- and tri-peptides via H +/peptide co-transporter-1 (PepT1). Aim: The aim of this study is to identify mechanisms of ileal adaptation after massive proximal enterectomy. Hypothesis: Ileal adaptation in uptake of peptides is mediated through upregulation of PepT1 gene expression. Study Design: Rats underwent 70% jejunoileal resection. Total mucosal cellular levels of messenger RNA (mRNA) and protein and transporter-mediated uptake per centimeter of the di-peptide glycyl-sarcosine (Gly-Sar) were compared in remnant ileum 1 and 4 weeks postoperatively to control and to 1-week sham laparotomy rats. Histomorphology, food consumption, and weights of rats were monitored. Results: After 70% resection, although mRNA per cell for PepT1 decreased at 1 week (p = 0. 002), expression of mRNA at 4 weeks and protein at 1 and 4 weeks in remnant ileum were unchanged (p > 0. 1). Ileal Gly-Sar uptake (V max-nanomoles per centimeter per minute, i. e., number of transporters per centimeter) increased at 1 and 4 weeks compared to control and 1-week sham (p < 0. 05 each); K m (i. e., transporter function) was unchanged. Villous heights (millimeters) in remnant ileum increased at 1- and 4-week time points over controls (0. 45 and 0. 57 vs 0. 21, resp; p < 0. 001). Conclusions: Ileal adaptation to proximal resection for peptide absorption occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of PepT1 mRNA or protein per enterocyte.

KW - Adaptation

KW - PepT1

KW - Peptide absorption

KW - Short bowel syndrome

KW - Small bowel resection

UR - http://www.scopus.com/inward/record.url?scp=80051918099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051918099&partnerID=8YFLogxK

U2 - 10.1007/s11605-011-1581-z

DO - 10.1007/s11605-011-1581-z

M3 - Article

VL - 15

SP - 1537

EP - 1547

JO - Journal of Gastrointestinal Surgery

JF - Journal of Gastrointestinal Surgery

SN - 1091-255X

IS - 9

ER -