Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation

Hisham G. Qandeel, Fernando Alonso, David J. Hernandez, Srivats Madhavan, Judith A. Duenes, Ye Zheng, Michael G. Sarr

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Protein absorption occurs as di- and tri-peptides via H +/peptide co-transporter-1 (PepT1). Aim: The aim of this study is to identify mechanisms of ileal adaptation after massive proximal enterectomy. Hypothesis: Ileal adaptation in uptake of peptides is mediated through upregulation of PepT1 gene expression. Study Design: Rats underwent 70% jejunoileal resection. Total mucosal cellular levels of messenger RNA (mRNA) and protein and transporter-mediated uptake per centimeter of the di-peptide glycyl-sarcosine (Gly-Sar) were compared in remnant ileum 1 and 4 weeks postoperatively to control and to 1-week sham laparotomy rats. Histomorphology, food consumption, and weights of rats were monitored. Results: After 70% resection, although mRNA per cell for PepT1 decreased at 1 week (p = 0. 002), expression of mRNA at 4 weeks and protein at 1 and 4 weeks in remnant ileum were unchanged (p > 0. 1). Ileal Gly-Sar uptake (V max-nanomoles per centimeter per minute, i. e., number of transporters per centimeter) increased at 1 and 4 weeks compared to control and 1-week sham (p < 0. 05 each); K m (i. e., transporter function) was unchanged. Villous heights (millimeters) in remnant ileum increased at 1- and 4-week time points over controls (0. 45 and 0. 57 vs 0. 21, resp; p < 0. 001). Conclusions: Ileal adaptation to proximal resection for peptide absorption occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of PepT1 mRNA or protein per enterocyte.

Original languageEnglish (US)
Pages (from-to)1537-1547
Number of pages11
JournalJournal of Gastrointestinal Surgery
Volume15
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

Symporters
Peptides
Ileum
Sarcosine
Messenger RNA
Proteins
Up-Regulation
Enterocytes
Laparotomy
Hyperplasia
Cell Proliferation
Gene Expression
Weights and Measures
Food
peptide permease

Keywords

  • Adaptation
  • PepT1
  • Peptide absorption
  • Short bowel syndrome
  • Small bowel resection

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Qandeel, H. G., Alonso, F., Hernandez, D. J., Madhavan, S., Duenes, J. A., Zheng, Y., & Sarr, M. G. (2011). Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation. Journal of Gastrointestinal Surgery, 15(9), 1537-1547. https://doi.org/10.1007/s11605-011-1581-z

Peptide Absorption After Massive Proximal Small Bowel Resection : Mechanisms of Ileal Adaptation. / Qandeel, Hisham G.; Alonso, Fernando; Hernandez, David J.; Madhavan, Srivats; Duenes, Judith A.; Zheng, Ye; Sarr, Michael G.

In: Journal of Gastrointestinal Surgery, Vol. 15, No. 9, 09.2011, p. 1537-1547.

Research output: Contribution to journalArticle

Qandeel, HG, Alonso, F, Hernandez, DJ, Madhavan, S, Duenes, JA, Zheng, Y & Sarr, MG 2011, 'Peptide Absorption After Massive Proximal Small Bowel Resection: Mechanisms of Ileal Adaptation', Journal of Gastrointestinal Surgery, vol. 15, no. 9, pp. 1537-1547. https://doi.org/10.1007/s11605-011-1581-z
Qandeel, Hisham G. ; Alonso, Fernando ; Hernandez, David J. ; Madhavan, Srivats ; Duenes, Judith A. ; Zheng, Ye ; Sarr, Michael G. / Peptide Absorption After Massive Proximal Small Bowel Resection : Mechanisms of Ileal Adaptation. In: Journal of Gastrointestinal Surgery. 2011 ; Vol. 15, No. 9. pp. 1537-1547.
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