Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage

C. J. Lai, Stephen C Ekker, P. A. Beachy, R. T. Moon

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The patterns of embryonic expression and the activities of Xenopus members of the hedgehog gene family are suggestive of roles in neural induction and patterning. We report that these hedgehog polypeptides undergo autoproteolytic cleavage. Injection into embryos of mRNAs encoding Xenopus banded-hedgehog (X-bhh) or the amino-terminal domain (N) demonstrates that the direct inductive activities of X-bhh are encoded by N. In addition, both N and X-bhh pattern neural tissue by elevating expression of anterior neural genes. Unexpectedly, an internal deletion of X-bhh (ΔN-C) was found to block the activity of X-bhh and N in explants and to reduce dorsoanterior structures in embryos. As elevated hedgehog activity increases the expression of anterior neural genes, and as ΔN-C reduces dorsoanterior structures, these complementary data support a role for hedgehog in neural induction and anteroposterior patterning.

Original languageEnglish (US)
Pages (from-to)2349-2360
Number of pages12
JournalDevelopment
Volume121
Issue number8
StatePublished - 1995
Externally publishedYes

Fingerprint

Ectoderm
Hedgehogs
Xenopus laevis
Xenopus
Embryonic Structures
Genes
Messenger RNA
Peptides
Injections

Keywords

  • Drosophila
  • Hedgehog
  • Neural induction
  • Protein processing
  • Spatial patterning

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage. / Lai, C. J.; Ekker, Stephen C; Beachy, P. A.; Moon, R. T.

In: Development, Vol. 121, No. 8, 1995, p. 2349-2360.

Research output: Contribution to journalArticle

@article{21bbf39592d94f3db8c0e50fd0014bcd,
title = "Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage",
abstract = "The patterns of embryonic expression and the activities of Xenopus members of the hedgehog gene family are suggestive of roles in neural induction and patterning. We report that these hedgehog polypeptides undergo autoproteolytic cleavage. Injection into embryos of mRNAs encoding Xenopus banded-hedgehog (X-bhh) or the amino-terminal domain (N) demonstrates that the direct inductive activities of X-bhh are encoded by N. In addition, both N and X-bhh pattern neural tissue by elevating expression of anterior neural genes. Unexpectedly, an internal deletion of X-bhh (ΔN-C) was found to block the activity of X-bhh and N in explants and to reduce dorsoanterior structures in embryos. As elevated hedgehog activity increases the expression of anterior neural genes, and as ΔN-C reduces dorsoanterior structures, these complementary data support a role for hedgehog in neural induction and anteroposterior patterning.",
keywords = "Drosophila, Hedgehog, Neural induction, Protein processing, Spatial patterning",
author = "Lai, {C. J.} and Ekker, {Stephen C} and Beachy, {P. A.} and Moon, {R. T.}",
year = "1995",
language = "English (US)",
volume = "121",
pages = "2349--2360",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "8",

}

TY - JOUR

T1 - Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage

AU - Lai, C. J.

AU - Ekker, Stephen C

AU - Beachy, P. A.

AU - Moon, R. T.

PY - 1995

Y1 - 1995

N2 - The patterns of embryonic expression and the activities of Xenopus members of the hedgehog gene family are suggestive of roles in neural induction and patterning. We report that these hedgehog polypeptides undergo autoproteolytic cleavage. Injection into embryos of mRNAs encoding Xenopus banded-hedgehog (X-bhh) or the amino-terminal domain (N) demonstrates that the direct inductive activities of X-bhh are encoded by N. In addition, both N and X-bhh pattern neural tissue by elevating expression of anterior neural genes. Unexpectedly, an internal deletion of X-bhh (ΔN-C) was found to block the activity of X-bhh and N in explants and to reduce dorsoanterior structures in embryos. As elevated hedgehog activity increases the expression of anterior neural genes, and as ΔN-C reduces dorsoanterior structures, these complementary data support a role for hedgehog in neural induction and anteroposterior patterning.

AB - The patterns of embryonic expression and the activities of Xenopus members of the hedgehog gene family are suggestive of roles in neural induction and patterning. We report that these hedgehog polypeptides undergo autoproteolytic cleavage. Injection into embryos of mRNAs encoding Xenopus banded-hedgehog (X-bhh) or the amino-terminal domain (N) demonstrates that the direct inductive activities of X-bhh are encoded by N. In addition, both N and X-bhh pattern neural tissue by elevating expression of anterior neural genes. Unexpectedly, an internal deletion of X-bhh (ΔN-C) was found to block the activity of X-bhh and N in explants and to reduce dorsoanterior structures in embryos. As elevated hedgehog activity increases the expression of anterior neural genes, and as ΔN-C reduces dorsoanterior structures, these complementary data support a role for hedgehog in neural induction and anteroposterior patterning.

KW - Drosophila

KW - Hedgehog

KW - Neural induction

KW - Protein processing

KW - Spatial patterning

UR - http://www.scopus.com/inward/record.url?scp=0029028990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029028990&partnerID=8YFLogxK

M3 - Article

VL - 121

SP - 2349

EP - 2360

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 8

ER -