Pathogenic human thyroglobulin peptides in HLA-DR3 transgenic mouse model of autoimmune thyroiditis

Jeffrey C. Flynn, Daniel J Mc Cormick, Vladimir Brusic, Qiang Wan, John C. Panos, Alvaro A. Giraldo, Chella S. David, Yi Chi M Kong

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To identify pathogenic epitopes on human thyroglobulin (hTg), a homodimer of 660 kDa, we have applied a computer-based algorithm to predict potential HLA-DR3-binding peptides and have tested them in DR3-transgenic mice. Of the 39 peptides selected, four stimulated a proliferative response from hTg-primed cells of DR3 + mice, but not DQ8 + mice. Of the four peptides, one, hTg2079, was consistently pathogenic. Thyroiditis was not only produced by adoptive transfer of hTg-primed, hTg2079-activated cells but also by direct immunization with the peptide. These results demonstrate the utility of using this computer-based algorithm with synthetic peptides to help identify pathogenic T cell epitopes on hTg.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalCellular Immunology
Volume229
Issue number2
DOIs
StatePublished - Jun 2004

Fingerprint

HLA-DR3 Antigen
Autoimmune Thyroiditis
Thyroglobulin
Transgenic Mice
Peptides
Thyroiditis
T-Lymphocyte Epitopes
Adoptive Transfer
Epitopes
Immunization

Keywords

  • Autoimmune thyroiditis
  • DR3-binding peptide
  • HLA-DR3
  • Thyroglobulin
  • Transgene

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Pathogenic human thyroglobulin peptides in HLA-DR3 transgenic mouse model of autoimmune thyroiditis. / Flynn, Jeffrey C.; Mc Cormick, Daniel J; Brusic, Vladimir; Wan, Qiang; Panos, John C.; Giraldo, Alvaro A.; David, Chella S.; Kong, Yi Chi M.

In: Cellular Immunology, Vol. 229, No. 2, 06.2004, p. 79-85.

Research output: Contribution to journalArticle

Flynn, Jeffrey C. ; Mc Cormick, Daniel J ; Brusic, Vladimir ; Wan, Qiang ; Panos, John C. ; Giraldo, Alvaro A. ; David, Chella S. ; Kong, Yi Chi M. / Pathogenic human thyroglobulin peptides in HLA-DR3 transgenic mouse model of autoimmune thyroiditis. In: Cellular Immunology. 2004 ; Vol. 229, No. 2. pp. 79-85.
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