Passive length-force properties of senescent diaphragm: Relationship with collagen characteristics

L. E. Gosselin, D. A. Martinez, A. C. Vailas, Gary C Sieck

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Abstract

The purpose of this study was to examine the effect of aging on collagen concentration and extent of nonreducible collagen cross-linking as well as with the passive length-force relationship of the diaphragm muscle. Midcostal diaphragm muscle strips from young (6 mo) and senescent (24 mo) Fischer 344 rats were perfused in a tissue bath containing mammalian Ringers solution (25°C) aerated with 95% O2-5% CO2. The segments were lengthened and shortened from 85 to 115% of optimal length (L(o)) at a constant velocity (0.6 L(o)/s), and the passive force was measured. Hydroxyproline (HYP) and the mature nonreducible collagen cross-link, hydroxylysylpyridinoline (HP), were measured by high-pressure liquid chromatography. The resting force at L(o) did not differ between young and senescent diaphragm muscles. However, the senescent diaphragm exhibited greater passive force compared with the young (P < 0.05) at lengths >110% of L(o). High-pressure liquid chromatography analysis revealed a higher concentration of HYP in the senescent compared with the young diaphragm (9.32 ± 0.83 and 6.59 ± 0.78 g HYP/mg dry wt, respectively; P < 0.05). Additionally, the content of HP was greater in the senescent compared with the young diaphragm (1.16 ± 0.05 and 0.91 ± 0.05 mol HP/mol collagen, respectively; P < 0.05). These results suggest that diaphragm collagen metabolism, maturation, and the passive length-force characteristics of the muscle are altered with senescence.

Original languageEnglish (US)
Pages (from-to)2680-2685
Number of pages6
JournalJournal of Applied Physiology
Volume76
Issue number6
StatePublished - 1994

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Keywords

  • connective tissue
  • extracellular matrix
  • skeletal muscle

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Gosselin, L. E., Martinez, D. A., Vailas, A. C., & Sieck, G. C. (1994). Passive length-force properties of senescent diaphragm: Relationship with collagen characteristics. Journal of Applied Physiology, 76(6), 2680-2685.