Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

Steven G Younkin, L. H. Younkin, M. Zoller, C. Rosenstein

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

Original languageEnglish (US)
Pages (from-to)192-194
Number of pages3
JournalBrain Research
Volume249
Issue number1
DOIs
StatePublished - Oct 7 1982
Externally publishedYes

Fingerprint

Distillation
Acetylcholinesterase
Vacuum
Diaphragm
Dry Ice
Electric Organ
Eels
Conditioned Culture Medium
Acetone
Muscles

Keywords

  • acetylcholinesterase
  • neuromuscular junction
  • organ culture
  • rat diaphragm
  • trophic factor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm. / Younkin, Steven G; Younkin, L. H.; Zoller, M.; Rosenstein, C.

In: Brain Research, Vol. 249, No. 1, 07.10.1982, p. 192-194.

Research output: Contribution to journalArticle

@article{a3b07d7bb80b4b2e8ba25ed5d94f3859,
title = "Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm",
abstract = "Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.",
keywords = "acetylcholinesterase, neuromuscular junction, organ culture, rat diaphragm, trophic factor",
author = "Younkin, {Steven G} and Younkin, {L. H.} and M. Zoller and C. Rosenstein",
year = "1982",
month = "10",
day = "7",
doi = "10.1016/0006-8993(82)90188-3",
language = "English (US)",
volume = "249",
pages = "192--194",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

AU - Younkin, Steven G

AU - Younkin, L. H.

AU - Zoller, M.

AU - Rosenstein, C.

PY - 1982/10/7

Y1 - 1982/10/7

N2 - Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

AB - Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

KW - acetylcholinesterase

KW - neuromuscular junction

KW - organ culture

KW - rat diaphragm

KW - trophic factor

UR - http://www.scopus.com/inward/record.url?scp=0019941928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019941928&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(82)90188-3

DO - 10.1016/0006-8993(82)90188-3

M3 - Article

VL - 249

SP - 192

EP - 194

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -