Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

S. G. Younkin, L. H. Younkin, M. Zoller, C. Rosenstein

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

Original languageEnglish (US)
Pages (from-to)192-194
Number of pages3
JournalBrain Research
Volume249
Issue number1
DOIs
StatePublished - Oct 7 1982

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Keywords

  • acetylcholinesterase
  • neuromuscular junction
  • organ culture
  • rat diaphragm
  • trophic factor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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