Partial conservation of functions between eukaryotic frataxin and the Escherichia coli frataxin homolog CyaY

Tibor Bedekovics, Gabriella B. Gajdos, Gyula Kispal, Grazia Isaya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Frataxin is a mitochondrial protein structurally conserved from bacteria to humans. Eukaryotic frataxins are known to be involved in the maintenance of mitochondrial iron balance via roles in iron delivery and iron detoxification. The prokaryotic frataxin homolog, CyaY, has been shown to bind and donate iron for the assembly of [2Fe-2S] clusters in vitro. However, in contrast to the severe phenotypes associated with the partial or complete loss of frataxin in humans and other eukaryotes, deletion of the cyaY gene does not cause any obvious alteration of iron balance in bacterial cells, an effect that probably reflects functional redundancy between CyaY and other bacterial proteins. To study CyaY function in a nonredundant setting, we have expressed a mitochondria-targeted form of CyaY in a Saccharomyces cerevisiae strain depleted of the endogenous yeast frataxin protein (yfh1Δ). We show that in this strain CyaY complements to a large extent the loss of iron-sulfur cluster enzyme activities and heme synthesis, and thereby maintains a nearly normal respiratory growth. In addition, CyaY effectively protects yfh1Δ from oxidative damage during treatment with hydrogen peroxide but is less efficient in detoxifying excess labile iron during aerobic growth.

Original languageEnglish (US)
Pages (from-to)1276-1284
Number of pages9
JournalFEMS Yeast Research
Volume7
Issue number8
DOIs
StatePublished - Dec 2007

Fingerprint

Iron
Escherichia coli
Fungal Proteins
Bacterial Proteins
Mitochondrial Proteins
Gene Deletion
Growth
frataxin
Eukaryota
Heme
Sulfur
Hydrogen Peroxide
Saccharomyces cerevisiae
Mitochondria
Maintenance
Bacteria
Phenotype
Enzymes

Keywords

  • CyaY
  • Frataxin
  • Heme
  • Iron chaperone
  • Iron homeostasis
  • Yfh1

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Partial conservation of functions between eukaryotic frataxin and the Escherichia coli frataxin homolog CyaY. / Bedekovics, Tibor; Gajdos, Gabriella B.; Kispal, Gyula; Isaya, Grazia.

In: FEMS Yeast Research, Vol. 7, No. 8, 12.2007, p. 1276-1284.

Research output: Contribution to journalArticle

Bedekovics, Tibor ; Gajdos, Gabriella B. ; Kispal, Gyula ; Isaya, Grazia. / Partial conservation of functions between eukaryotic frataxin and the Escherichia coli frataxin homolog CyaY. In: FEMS Yeast Research. 2007 ; Vol. 7, No. 8. pp. 1276-1284.
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