partial characterization of an insulin-dependent serum factor that regulates ornithine decarboxylase in skeletal muscle

Cheryl A Conover, S. J. Rozovski, N. B. Ruderman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have previously shown that a factor(s) in rat serum induces ornithine decarboxylase (ODC) in incubated muscle and that its activity is diminished in sera from diabetic rats. To characterize this factor further, we have studied some of its physicochemical and biological properties. As judged from its ability to induce ODC in the incubated rat soleus muscle, the factor is protease-sensitive and both heat- and acid-stable. In untreated whole serum its activity is associated with a high-molecular-weight fraction whereas after boiling at pH 5.5, activity is principally in a fraction with a molecular weight between 3500 and 12,000 daltons. The activity of the factor is diminished in hypophysectomized, starved, and aged as well as diabetic rats. In diabetic rat serum it is restored to normal by the addition of a purified somatomedin, multiplication-stimulating activity. These findings suggest that the 'ODC inducing factor' is a low-molecular-weight peptide and that it has many of the characteristics of a somatomedin.

Original languageEnglish (US)
Pages (from-to)500-505
Number of pages6
JournalDiabetes
Volume31
Issue number6 I
StatePublished - 1982
Externally publishedYes

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Ornithine Decarboxylase
Skeletal Muscle
Insulin
Serum
Molecular Weight
Somatomedins
Insulin-Like Growth Factor II
Peptide Hydrolases
Hot Temperature
Muscles
Peptides
Acids

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

partial characterization of an insulin-dependent serum factor that regulates ornithine decarboxylase in skeletal muscle. / Conover, Cheryl A; Rozovski, S. J.; Ruderman, N. B.

In: Diabetes, Vol. 31, No. 6 I, 1982, p. 500-505.

Research output: Contribution to journalArticle

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