Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells

Joseph Bidwell, Andre J van Wijnen, Chaitali Banerjee, Edward Fey, Harold Merriman, Slheldon Plenman, Jlanet Sltein, Jane Lian, Gary Stein

Research output: Contribution to journalArticle

Abstract

PTH is a mediator of skeletal development and remodeling that influences gene expression in osteoblastic cells. It is well established that PTH modulates the activity of membrane-associated second messenger signal transduction pathways. In these studies we have addressed the potential contribution of components of cell structure to the integration of PTH-related regulatory signals that influence the expression of bone cell genes. Chronic treatment of ROS 17/2.8 rat osteosarcoma cells with PTH is accompanied by changes in gene expression that are at least in part transcriptionally controlled. To explore the involvement of nuclear architecture in PTH-responsive modifications in gene expression, we investigated changes in the nuclear matrix after PTH treatment. Consistent with a role for the nuclear matrix in determining spatial organization and topology of chromatin as well as in the localization and targeting of transcription factors, we observed PTH-associated changes in a 200-kilodalton nuclear matrix protein in response to PTH. A significant down-regulation of synthesis was observed when nuclear matrix proteins were resolved electrophoretically in two-dimensional gels. This protein was restricted to the nuclear matrix and was not detected in the chromatin or cytoskeletal cellular fractions. These alterations in nuclear matrix proteins that occur after PTH treatment in osteosarcoma cells were phenotype related. They did not occur in UMR-106 POL or H4 hepatoma cells. Our findings support a role for the nuclear matrix in transducing PTH-mediated regulatory signals to facilitate the extent to which genes in osteoblasts are transcribed.

Original languageEnglish (US)
Pages (from-to)1738-1744
Number of pages7
JournalEndocrinology
Volume134
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

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Nuclear Matrix
Osteosarcoma
Nuclear Matrix-Associated Proteins
Gene Expression
Chromatin
Second Messenger Systems
Cellular Structures
Osteoblasts
Genes
Hepatocellular Carcinoma
Signal Transduction
Transcription Factors
Down-Regulation
Gels
Phenotype
Bone and Bones
Membranes
Proteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Bidwell, J., van Wijnen, A. J., Banerjee, C., Fey, E., Merriman, H., Plenman, S., ... Stein, G. (1994). Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells. Endocrinology, 134(4), 1738-1744. https://doi.org/10.1210/endo.134.4.8137738

Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells. / Bidwell, Joseph; van Wijnen, Andre J; Banerjee, Chaitali; Fey, Edward; Merriman, Harold; Plenman, Slheldon; Sltein, Jlanet; Lian, Jane; Stein, Gary.

In: Endocrinology, Vol. 134, No. 4, 1994, p. 1738-1744.

Research output: Contribution to journalArticle

Bidwell, J, van Wijnen, AJ, Banerjee, C, Fey, E, Merriman, H, Plenman, S, Sltein, J, Lian, J & Stein, G 1994, 'Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells', Endocrinology, vol. 134, no. 4, pp. 1738-1744. https://doi.org/10.1210/endo.134.4.8137738
Bidwell J, van Wijnen AJ, Banerjee C, Fey E, Merriman H, Plenman S et al. Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells. Endocrinology. 1994;134(4):1738-1744. https://doi.org/10.1210/endo.134.4.8137738
Bidwell, Joseph ; van Wijnen, Andre J ; Banerjee, Chaitali ; Fey, Edward ; Merriman, Harold ; Plenman, Slheldon ; Sltein, Jlanet ; Lian, Jane ; Stein, Gary. / Parathyroid-responsive modifications in the nuclear matrix of ros 17/2.8 Rat osteosarcoma cells. In: Endocrinology. 1994 ; Vol. 134, No. 4. pp. 1738-1744.
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