Paraoxon toxicity is not potentiated by prior reduction in blood acetylcholinesterase

S. Padilla, V. C. Moser, C. N. Pope, W. S. Brimijoin

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The role of blood acetylcholinesterase in moderating the effects of organophosphate challenge in rats was tested. Adult male rats (n = 42) were injected (iv) either with monoclonal antibodies (MAb) to rat acetylcholinesterase (EC 3.1.1.7; AChE) or normal mouse IgG (controls). Two days later, the rats were injected (sc) with either a mild (0.17 mg/kg) or moderate dosage (0.34 mg/kg) of paraoxon or with vehicle. Neurological integrity was assessed by a functional observational battery followed by motor activity, 3 to 4 hr after dosing. Blood, brain, and diaphragm tissues were then collected for determination of AChE activity. MAb treatment reduced whole blood and plasma AChE activity by 32 and 90%, respectively, but did not affect neurobehavioral parameters or the AChE activity of brain or diaphragm. The paraoxon challenge produced dose-related neurobehavioral changes and inhibition of brain and diaphragm AChE activity to the same extent in IgG- and MAb-treated rats. Thus, significant loss in blood AChE alone produced no detectable neurobehavioral deficits and did not alter the subsequent responses to paraoxon challenge.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalToxicology and Applied Pharmacology
Volume117
Issue number1
DOIs
StatePublished - Nov 1992

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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