Paracrine effects of living human bone particles on the osteogenic differentiation of mesenchymal stem cells

Aysegul Atasoy-Zeybek, A. Ivkovic, T. Beyzadeoglu, A. Onal, C. H. Evans, G. T. Kose

Research output: Contribution to journalArticle

Abstract

Bone autografting remains the clinical treatment of choice for resolving problematic fractures. The precise mechanisms through which the autograft promotes bone healing are unknown. The present study examined the hypothesis that cells within the autograft secrete osteogenic factors promoting the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. Particles of human bone (“chips”) were recovered at the time of joint replacement surgery and placed in culture. Then, conditioned media were added to cultures of human, adipose-derived MSCs under both basal and osteogenic conditions. Contrary to expectation, medium conditioned by bone chips reduced the expression of alkaline phosphatase and strongly inhibited mineral deposition by MSCs cultured in osteogenic medium. Real time PCR revealed the inhibition of collagen type I alpha 1 chain (Col1A1) and osteopontin (OPN) expression. These data indicated that the factors secreted by bone chips inhibited the osteogenic differentiation of MSCs. However, in late cultures, bone morphogenetic protein-2 (BMP-2) expression was stimulated, suggesting the possibility of a delayed, secondary osteogenic effect.

Original languageEnglish (US)
Pages (from-to)14-22
Number of pages9
JournalEuropean Cells and Materials
Volume38
DOIs
StatePublished - Dec 2019

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Stem cells
Mesenchymal Stromal Cells
Bone
Bone and Bones
Autografts
Conditioned Culture Medium
Cell culture
Replacement Arthroplasties
Bone Morphogenetic Protein 2
Osteopontin
Autologous Transplantation
Osteoblasts
Surgery
Phosphatases
Minerals
Alkaline Phosphatase
Real-Time Polymerase Chain Reaction
Collagen
Proteins
Therapeutics

Keywords

  • Autograft
  • Bone chips
  • Fracture healing
  • Mesenchymal stem cell
  • Osteogenesis

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Paracrine effects of living human bone particles on the osteogenic differentiation of mesenchymal stem cells. / Atasoy-Zeybek, Aysegul; Ivkovic, A.; Beyzadeoglu, T.; Onal, A.; Evans, C. H.; Kose, G. T.

In: European Cells and Materials, Vol. 38, 12.2019, p. 14-22.

Research output: Contribution to journalArticle

Atasoy-Zeybek, Aysegul ; Ivkovic, A. ; Beyzadeoglu, T. ; Onal, A. ; Evans, C. H. ; Kose, G. T. / Paracrine effects of living human bone particles on the osteogenic differentiation of mesenchymal stem cells. In: European Cells and Materials. 2019 ; Vol. 38. pp. 14-22.
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