TY - JOUR
T1 - Pancreatic gene expression during the initiation of acute pancreatitis
T2 - Identification of EGR-1 as a key regulator
AU - Ji, Baoan
AU - Chen, Xue Qing
AU - Misek, David E.
AU - Kuick, Rork
AU - Hanash, Samir
AU - Ernst, Steve
AU - Najarian, Rebecca
AU - Logsdon, Craig D.
PY - 2003/10
Y1 - 2003/10
N2 - We hypothesized that genes expressed in pancreatic acinar cells during the initiation of acute pancreatitis determine the severity of the disease. Therefore, we utilized microarrays to identify those genes commonly induced in rat pancreatic acinar cells within 1-4 h in two in vivo models, caerulein and taurocholate administration. This strategy yielded 51 known genes representing a complex array of molecules, including those that are likely to either reduce or increase the severity of the disease. Novel genes identified in the current study included ATF3, BRF1, C/EBPβ, CGRP, EGR-1, ephrinal, villin2, ferredoxin, latexin, lipocalin, MKP-1, NGFI-B, RhoA, tissue factor (TF), and syndecan. To validate these microarray results, the role of EGR-1 was further investigated using quantitative RT-PCR, Western blotting, and immunocytochemistry. EGR-1 expression occurred within acinar cells and correlated with the development of caerulein-induced acute pancreatitis in rats. Furthermore, the levels of the inflammation-related genes MCP-1, PAI, TF, IL-6, and ICAM-1 and the extent of lung inflammation were reduced during the initiation of caerulein-induced acute pancreatitis in EGR-1-deficient mice. Thus this study identified EGR-1 and several other novel genes likely to be important in the development and severity of acute pancreatitis.
AB - We hypothesized that genes expressed in pancreatic acinar cells during the initiation of acute pancreatitis determine the severity of the disease. Therefore, we utilized microarrays to identify those genes commonly induced in rat pancreatic acinar cells within 1-4 h in two in vivo models, caerulein and taurocholate administration. This strategy yielded 51 known genes representing a complex array of molecules, including those that are likely to either reduce or increase the severity of the disease. Novel genes identified in the current study included ATF3, BRF1, C/EBPβ, CGRP, EGR-1, ephrinal, villin2, ferredoxin, latexin, lipocalin, MKP-1, NGFI-B, RhoA, tissue factor (TF), and syndecan. To validate these microarray results, the role of EGR-1 was further investigated using quantitative RT-PCR, Western blotting, and immunocytochemistry. EGR-1 expression occurred within acinar cells and correlated with the development of caerulein-induced acute pancreatitis in rats. Furthermore, the levels of the inflammation-related genes MCP-1, PAI, TF, IL-6, and ICAM-1 and the extent of lung inflammation were reduced during the initiation of caerulein-induced acute pancreatitis in EGR-1-deficient mice. Thus this study identified EGR-1 and several other novel genes likely to be important in the development and severity of acute pancreatitis.
KW - Acute respiratory distress syndrome
KW - Inflammation
KW - Pancreas
KW - Pancreatic acinar cell
KW - Real-time quantitative reverse transcription-polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0242365486&partnerID=8YFLogxK
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U2 - 10.1152/physiolgenomics.00174.2002
DO - 10.1152/physiolgenomics.00174.2002
M3 - Article
C2 - 12709512
AN - SCOPUS:0242365486
SN - 1531-2267
VL - 14
SP - 59
EP - 72
JO - Physiological Genomics
JF - Physiological Genomics
ER -