Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression

Long Jin, Bernd W. Scheithauer, William Francis Young, Dudley H. Davis, George G. Klee, Ricardo V. Lloyd

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pancreastatin, a carboxyl-terminal amidated peptide derived from chromogranin (Cg)A, inhibits secretion of insulin and parathyroid hormone. Our recent studies found significant amounts of immunoreactive pancreastatin in all pituitary adenomas except prolactin adenomas. To analyze the effects of pancreastatin on pituitary cell function, 17 cultured pituitary adenomas were examined for immunoreactive pancreastatin and pancreastatin secretion by the tumors. The effects of pancreastatin on pituitary hormone secretion and on pituitary hormone (follicle-stimulating hormone and prolactin), CgA, and CgB mRNA levels were also examined. Immunoreactive pancreastatin and CgA were present diffusely in gonadotroph and null cell adenomas, but only a few prolactin adenoma cells expressed pancreastatin or CgA. When cells were treated with hypothalamic peptides, gonadotroph adenomas were the only group that released increased amounts of pancreastatin in response to gonadotropin-releasing hormone (10-7 mol/L). Pancreastatin (10-7 mol/L) treatment did not stimulate pituitary hormone secretion significantly. In situ hybridization analyses showed that gonadotropin-releasing hormone and pancreastatin treatment led to significant increases in CgB and follicle- stimulating hormone mRNAs in gonadotroph adenomas, whereas CgA mRNA levels did not change significantly. These results show that there is a differential distribution of pancreastatin secretion in pituitary adenomas and that the hypothalamic hormone gonadotropin- releasing hormone and the CgA-derived peptide pancreastatin can regulate CgB mRNA in gonadotroph adenomas, suggesting an autocrine effect of pancreastatin on pituitary tumor function.

Original languageEnglish (US)
Pages (from-to)2057-2066
Number of pages10
JournalAmerican Journal of Pathology
Volume148
Issue number6
StatePublished - Jun 1996

Fingerprint

Chromogranin B
Pituitary Neoplasms
Messenger RNA
Adenoma
Gonadotrophs
Pituitary Hormones
Gonadotropin-Releasing Hormone
Prolactin
Follicle Stimulating Hormone
pancreastatin
Peptides
Hypothalamic Hormones
Chromogranin A
Null Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Jin, L., Scheithauer, B. W., Young, W. F., Davis, D. H., Klee, G. G., & Lloyd, R. V. (1996). Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression. American Journal of Pathology, 148(6), 2057-2066.

Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression. / Jin, Long; Scheithauer, Bernd W.; Young, William Francis; Davis, Dudley H.; Klee, George G.; Lloyd, Ricardo V.

In: American Journal of Pathology, Vol. 148, No. 6, 06.1996, p. 2057-2066.

Research output: Contribution to journalArticle

Jin, L, Scheithauer, BW, Young, WF, Davis, DH, Klee, GG & Lloyd, RV 1996, 'Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression', American Journal of Pathology, vol. 148, no. 6, pp. 2057-2066.
Jin, Long ; Scheithauer, Bernd W. ; Young, William Francis ; Davis, Dudley H. ; Klee, George G. ; Lloyd, Ricardo V. / Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression. In: American Journal of Pathology. 1996 ; Vol. 148, No. 6. pp. 2057-2066.
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