Padé-Laplace method for the analysis of time-resolved fluorescence decay curves

Z. Bajzer, J. C. Sharp, S. S. Sedarous, F. G. Prendergast

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The interpretation of fluorescence intensity decay times in terms of protein structure and dynamics depends on the accuracy and sensitivity of the methods used for data analysis. The are many methods available for the analysis of fluorescence decay data, but justification for choosing any one of them is unclear. In this paper we generalize the recently proposed Padé-Laplace method [45] to include deconvolution with respect to the instrument response function. In this form the method can be readily applied to the analysis of time-correlated single photon counting data. By extensive simulations we have shown that the Padé-Laplace method provides more accurate results than the standard least squares method with iterative reconvolution under the condition of closely spaced lifetimes. The application of the Padé-Laplace method to several experimental data sets yielded results consistent with those obtained by use of the least squares analysis.

Original languageEnglish (US)
Pages (from-to)101-115
Number of pages15
JournalEuropean Biophysics Journal
Volume18
Issue number2
DOIs
StatePublished - Mar 1 1990

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Keywords

  • Fluorescence lifetime
  • Padé approximants

ASJC Scopus subject areas

  • Biophysics

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